Sulfatase-activated fluorophores for rapid discrimination of mycobacterial species and strains

Kimberly Beatty, Monique Williams, Brian L. Carlson, Benjamin M. Swarts, Robin M. Warren, Paul D. Van Helden, Carolyn R. Bertozzi

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

Most current diagnostic tests for tuberculosis do not reveal the species or strain of pathogen causing pulmonary infection, which can lead to inappropriate treatment regimens and the spread of disease. Here, we report an assay for mycobacterial strain assignment based on genetically conserved mycobacterial sulfatases. We developed a sulfatase-activated probe, 7-hydroxy-9H-(1,3- dichloro-9,9-dimethylacridin-2-one)-sulfate, that detects enzyme activity in native protein gels, allowing the rapid detection of sulfatases in mycobacterial lysates. This assay revealed that mycobacterial strains have distinct sulfatase fingerprints that can be used to judge both the species and lineage. Our results demonstrate the potential of enzyme-activated probes for rapid pathogen discrimination for infectious diseases.

Original languageEnglish (US)
Pages (from-to)12911-12916
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume110
Issue number32
DOIs
Publication statusPublished - Aug 6 2013

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Keywords

  • Chemical biology
  • Enzyme assay
  • Fluorescence
  • Hydrolase
  • Mycobacterium tuberculosis

ASJC Scopus subject areas

  • General

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