TY - JOUR
T1 - Subunit organization and structure in the F0 sector of Escherichia coli F1F0 ATP synthase
AU - Fillingame, R. H.
AU - Jones, P. C.
AU - Jiang, W.
AU - Valiyaveetil, F. I.
AU - Dmitriev, O. Y.
N1 - Funding Information:
Much of the work described here could not be interpreted without the atomic resolution model of subunit c. We acknowledge Dr. Mark Girvin (Albert Einstein College of Medicine, Bronx, NY) as the primary contributor to the completion of that structure. The work described in the author's laboratory was supported by United States Public Health Service Grant GM-23105 and a grant from the Human Frontiers Science Program. The National Magnetic Resonance Facility at Madison was used in these studies.
PY - 1998/6/10
Y1 - 1998/6/10
N2 - In this review, we summarize recent work from our laboratory which establishes the topology and nearest neighbor organization of subunits in the F0 sector of the H+ transporting ATP synthase of Escherichia coli. The E. coli F0 sector is composed of three subunits in an a1b2c12 stoichiometric ratio. Crosslinking experiments with genetically introduced Cys establish a ring-like organization of the 12 c subunits with subunits a and b lying to the outside of the ring. The results are interpreted using an atomic resolution structural model of monomeric subunit c in a chloroform- methanol-water (4:4:1, v/v/v) solution, derived by heteronuclear NMR (M.E. Girvin, F. Abildgaard, V. Rastogi, J. Markley, R.H. Fillingame, in press). The crosslinking results validate many predictions of the structural model and confirm a front-to-back-type packing of two subunit c into a functional dimer, as was first predicted from genetic studies. Aspartyl-61, the proton translocating residue, lies at the center of the four transmembrane helices of the functional dimer, rather than at the periphery of the subunit c ring. Subunit a is shown to fold with five transmembrane helices, and a functionally important interaction of transmembrane helix-4 with transmembrane helix-2 of subunit c is established. The single transmembrane helices of the two subunit b dimerize in the membrane. The structure of the transmembrane segment of subunit b is predicted from the NMR structure of the monomeric peptide.
AB - In this review, we summarize recent work from our laboratory which establishes the topology and nearest neighbor organization of subunits in the F0 sector of the H+ transporting ATP synthase of Escherichia coli. The E. coli F0 sector is composed of three subunits in an a1b2c12 stoichiometric ratio. Crosslinking experiments with genetically introduced Cys establish a ring-like organization of the 12 c subunits with subunits a and b lying to the outside of the ring. The results are interpreted using an atomic resolution structural model of monomeric subunit c in a chloroform- methanol-water (4:4:1, v/v/v) solution, derived by heteronuclear NMR (M.E. Girvin, F. Abildgaard, V. Rastogi, J. Markley, R.H. Fillingame, in press). The crosslinking results validate many predictions of the structural model and confirm a front-to-back-type packing of two subunit c into a functional dimer, as was first predicted from genetic studies. Aspartyl-61, the proton translocating residue, lies at the center of the four transmembrane helices of the functional dimer, rather than at the periphery of the subunit c ring. Subunit a is shown to fold with five transmembrane helices, and a functionally important interaction of transmembrane helix-4 with transmembrane helix-2 of subunit c is established. The single transmembrane helices of the two subunit b dimerize in the membrane. The structure of the transmembrane segment of subunit b is predicted from the NMR structure of the monomeric peptide.
KW - ATP synthase
KW - Crosslinking
KW - F structure
KW - NMR
KW - Proton translocation
KW - Subunit c
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U2 - 10.1016/S0005-2728(98)00053-X
DO - 10.1016/S0005-2728(98)00053-X
M3 - Article
C2 - 9693732
AN - SCOPUS:0032314695
SN - 0005-2728
VL - 1365
SP - 135
EP - 142
JO - Biochimica et Biophysica Acta - Bioenergetics
JF - Biochimica et Biophysica Acta - Bioenergetics
IS - 1-2
ER -