Subtle sequence variations alter tripartite complex kinetics and G-quadruplex dynamics in RNA aptamer Broccoli

Jonathan C. Savage, Monika A. Davare, Ujwal Shinde

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Though extensively utilized, the fluorescent RNA aptamer Broccoli is poorly characterized with an unknown structure. Spectroscopic and kinetic investigations of tripartite complex formation reveal surprising differences between Broccoli and Spinach aptamers despite extreme sequence conservation. Our studies highlight how subtle sequence variations impart functional consequences of G-quadruplex-cation interactions in RNA.

Original languageEnglish (US)
Pages (from-to)2634-2637
Number of pages4
JournalChemical Communications
Volume56
Issue number17
DOIs
StatePublished - Feb 28 2020

ASJC Scopus subject areas

  • Catalysis
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

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