Subtle changes in endochin-like quinolone structure alter the site of inhibition within the cytochrome bc1 complex of plasmodium falciparum

Allison M. Stickles, Mariana Justino De Almeida, Joanne M. Morrisey, Kayla A. Sheridan, Isaac P. Forquer, Aaron Nilsen, Rolf W. Winter, Jeremy N. Burrows, David A. Fidock, Akhil B. Vaidya, Michael K. Riscoe

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

The cytochrome bc1 complex (cyt bc1) is the third component of the mitochondrial electron transport chain and is the target of several potent antimalarial compounds, including the naphthoquinone atovaquone (ATV) and the 4(1H)-quinolone ELQ-300. Mechanistically, cyt bc1 facilitates the transfer of electrons from ubiquinol to cytochrome c and contains both oxidative (Qo) and reductive (Qi) catalytic sites that are amenable to small-molecule inhibition. Although many antimalarial compounds, including ATV, effectively target the Qo site, it has been challenging to design selective Qi site inhibitors with the ability to circumvent clinical ATV resistance, and little is known about how chemical structure contributes to site selectivity within cyt bc1. Here, we used the proposed Qi site inhibitor ELQ-300 to generate a drug-resistant Plasmodium falciparum clone containing an I22L mutation at the Qi region of cyt b. Using this D1 clone and the Y268S Qo mutant strain, P. falciparum Tm90-C2B, we created a structureactivity map of Qi versus Qo site selectivity for a series of endochin-like 4(1H)-quinolones (ELQs). We found that Qi site inhibition was associated with compounds containing 6-position halogens or aryl 3-position side chains, while Qo site inhibition was favored by 5,7-dihalogen groups or 7-position substituents. In addition to identifying ELQ-300 as a preferential Qi site inhibitor, our data suggest that the 4(1H)-quinolone scaffold is compatible with binding to either site of cyt bc1 and that minor chemical changes can influence Qo or Qi site inhibition by the ELQs.

Original languageEnglish (US)
Pages (from-to)1977-1982
Number of pages6
JournalAntimicrobial agents and chemotherapy
Volume59
Issue number4
DOIs
StatePublished - Apr 1 2015

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

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