Substrate competition between DNase I and anti-DNA antibody

Michael Liebling, Eugene V. Barnett

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Sera from rabbits immunized with DNA and patients known to have SLE were shown to inhibit the digestion of 125I-labeled ss-DNA by pancreatic DNase I. Similarly, DNase present in the serum of a patient with SLE was found to digest the radio-labeled DNA at one-sixth the rate of an equal quantity of DNase present in NHS. The inhibition was shown to be due to isolated anti-DNA antibody in both the immunized rabbit sera and the SLE sera. The type of inhibition involved was demonstrated to be of the competitive variety in which the binding of antibody to DNA presumably blocks enzyme access to the substrate. The possible pathogenetic implications of these findings are discussed.

Original languageEnglish (US)
Pages (from-to)80-89
Number of pages10
JournalClinical Immunology and Immunopathology
Volume8
Issue number1
DOIs
StatePublished - 1977
Externally publishedYes

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Deoxyribonuclease I
Antinuclear Antibodies
Deoxyribonucleases
DNA
Serum
Rabbits
Radio
Digestion
Antibodies
Enzymes

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Pathology and Forensic Medicine

Cite this

Substrate competition between DNase I and anti-DNA antibody. / Liebling, Michael; Barnett, Eugene V.

In: Clinical Immunology and Immunopathology, Vol. 8, No. 1, 1977, p. 80-89.

Research output: Contribution to journalArticle

Liebling, Michael ; Barnett, Eugene V. / Substrate competition between DNase I and anti-DNA antibody. In: Clinical Immunology and Immunopathology. 1977 ; Vol. 8, No. 1. pp. 80-89.
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