Abstract
Objective: To characterize GFP-expressing cells in the striatum of Cb6-Tg(Gad1-EGFP)G42Zjh/J mice, in which the Gad1 (also referred to as GAD67) promoter drives GFP expression (Gad1-GFP mouse). Background: GFP-expressing cells of the GAD1-GFP mouse have been described to be a population of parvalbumin-positive basket interneurons residing in the cerebral cortex and the cerebellum. However, the cells in the dorsal striatum of these mice have not been characterized. Methods: Using a combination of immunohistochemistry, electrophysiology, DiI labeling, and retrograde tracing, we investigated the phenotypes of GFP-expressing cells in the GAD1-GFP mice. Results: A small number of striatal neurons express GFP in these mice. In the mature striatum, these cells are preferentially located in the lateral striatum with a strong expression in the lateral striatal streak. The GAD1-GFP positive neurons are distinct from the standard fast-spiking and low-threshold-spiking GAD-67 expressing striatal interneurons and appear to be a subset of medium spiny neurons. These neurons are generally colocalized with striosomal markers such as dynorphin, mu-opioid receptors, as well as CB1 and calretinin-immunopositive fibers. Striatal Gad1-GFP neurons can be separated into two groups based on the shape of the somata and patterns of action potential firing. Retrograde labeling indicated that a proportion of these cells are projection neurons. Conclusions: The examination of GAD1-GFP cells in these mice revealed two subpopulations of ventral striosomal striatal medium spiny neurons, based on morphology, patch-matrix segregation and membrane properties.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 201-211 |
| Number of pages | 11 |
| Journal | Basal Ganglia |
| Volume | 1 |
| Issue number | 4 |
| DOIs | |
| State | Published - Nov 2011 |
| Externally published | Yes |
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Keywords
- BAC transgenic
- Basal Ganglia
- GABA
- Medium spiny neuron
- Patch
ASJC Scopus subject areas
- Clinical Neurology
- Neurology
Cite this
Subsets of spiny striosomal striatal neurons revealed in the Gad1-GFP BAC transgenic mouse. / Cuzon Carlson, Verginia; Mathur, Brian N.; Davis, Margaret I.; Lovinger, David M.
In: Basal Ganglia, Vol. 1, No. 4, 11.2011, p. 201-211.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Subsets of spiny striosomal striatal neurons revealed in the Gad1-GFP BAC transgenic mouse
AU - Cuzon Carlson, Verginia
AU - Mathur, Brian N.
AU - Davis, Margaret I.
AU - Lovinger, David M.
PY - 2011/11
Y1 - 2011/11
N2 - Objective: To characterize GFP-expressing cells in the striatum of Cb6-Tg(Gad1-EGFP)G42Zjh/J mice, in which the Gad1 (also referred to as GAD67) promoter drives GFP expression (Gad1-GFP mouse). Background: GFP-expressing cells of the GAD1-GFP mouse have been described to be a population of parvalbumin-positive basket interneurons residing in the cerebral cortex and the cerebellum. However, the cells in the dorsal striatum of these mice have not been characterized. Methods: Using a combination of immunohistochemistry, electrophysiology, DiI labeling, and retrograde tracing, we investigated the phenotypes of GFP-expressing cells in the GAD1-GFP mice. Results: A small number of striatal neurons express GFP in these mice. In the mature striatum, these cells are preferentially located in the lateral striatum with a strong expression in the lateral striatal streak. The GAD1-GFP positive neurons are distinct from the standard fast-spiking and low-threshold-spiking GAD-67 expressing striatal interneurons and appear to be a subset of medium spiny neurons. These neurons are generally colocalized with striosomal markers such as dynorphin, mu-opioid receptors, as well as CB1 and calretinin-immunopositive fibers. Striatal Gad1-GFP neurons can be separated into two groups based on the shape of the somata and patterns of action potential firing. Retrograde labeling indicated that a proportion of these cells are projection neurons. Conclusions: The examination of GAD1-GFP cells in these mice revealed two subpopulations of ventral striosomal striatal medium spiny neurons, based on morphology, patch-matrix segregation and membrane properties.
AB - Objective: To characterize GFP-expressing cells in the striatum of Cb6-Tg(Gad1-EGFP)G42Zjh/J mice, in which the Gad1 (also referred to as GAD67) promoter drives GFP expression (Gad1-GFP mouse). Background: GFP-expressing cells of the GAD1-GFP mouse have been described to be a population of parvalbumin-positive basket interneurons residing in the cerebral cortex and the cerebellum. However, the cells in the dorsal striatum of these mice have not been characterized. Methods: Using a combination of immunohistochemistry, electrophysiology, DiI labeling, and retrograde tracing, we investigated the phenotypes of GFP-expressing cells in the GAD1-GFP mice. Results: A small number of striatal neurons express GFP in these mice. In the mature striatum, these cells are preferentially located in the lateral striatum with a strong expression in the lateral striatal streak. The GAD1-GFP positive neurons are distinct from the standard fast-spiking and low-threshold-spiking GAD-67 expressing striatal interneurons and appear to be a subset of medium spiny neurons. These neurons are generally colocalized with striosomal markers such as dynorphin, mu-opioid receptors, as well as CB1 and calretinin-immunopositive fibers. Striatal Gad1-GFP neurons can be separated into two groups based on the shape of the somata and patterns of action potential firing. Retrograde labeling indicated that a proportion of these cells are projection neurons. Conclusions: The examination of GAD1-GFP cells in these mice revealed two subpopulations of ventral striosomal striatal medium spiny neurons, based on morphology, patch-matrix segregation and membrane properties.
KW - BAC transgenic
KW - Basal Ganglia
KW - GABA
KW - Medium spiny neuron
KW - Patch
UR - http://www.scopus.com/inward/record.url?scp=83155180231&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=83155180231&partnerID=8YFLogxK
U2 - 10.1016/j.baga.2011.11.002
DO - 10.1016/j.baga.2011.11.002
M3 - Article
AN - SCOPUS:83155180231
VL - 1
SP - 201
EP - 211
JO - Basal Ganglia
JF - Basal Ganglia
SN - 2210-5336
IS - 4
ER -