SUBRETINAL CELL-BASED THERAPY

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Purpose: To develop a novel surgical approach to provide consistent delivery of cell suspension into the subretinal space without cell leakage into the vitreous. Methods: Cell viability was assessed following mock injections to determine the optimal size cannula for delivery of the cells. A pars plana without vitrectomy approach was used to create a subretinal bleb with balanced salt solution using a 41-gauge cannula. GFP-labeled retinal pigment epithelium cells were injected through transretinal (n = 8) and transscleral (n = 16) injection approaches. Optical coherence tomography, fundus photography and autofluorescence, and histological analysis were used to evaluate surgical success. Results: The 30-gauge cannula yielded the highest recovery of cells with highest viability. The transretinal approach consistently resulted in transplanted cells in the vitreous, with some cells coming to rest on the inner limiting membrane. Conversely, the transscleral approach resulted in transplantation of cells into the subretinal space in 100% of cases. Histological analysis confirmed these results. Conclusion: We have developed a novel surgical approach that resulted in encapsulation of transplanted cells into the subretinal space with a 100% success rate. This approach will provide a useful tool for further cell transplantation study and may provide an approach for clinical application of delivering cells to the subretinal space.

Original languageEnglish (US)
Pages (from-to)2162-2166
Number of pages5
JournalRetina
Volume37
Issue number11
DOIs
StatePublished - Nov 1 2017

Keywords

  • cell transplantation
  • detachment
  • subretinal injection
  • surgical technique

ASJC Scopus subject areas

  • Ophthalmology

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