Subperineurial demyelination associated with reduced nerve blood flow and oxygen tension after epineurial vascular stripping

R. R. Myers, H. M. Heckman, James Galbraith, H. C. Powell

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Using laser Doppler measurements of nerve blood flow and electron microscopy, we determined that removal of the vasa nervorum from the surface of rat peripheral nerve results in an immediate 58.4% ± (SD) 12.6% reduction in nerve blood flow (p <0.017) and subsequent subperineurial demyelination. To further assess the role of ischemia in demyelination, a second group of Sprague-Dawley rats (250 to 300 gm) was anesthetized and oxygen tensions were recorded with platinum microelectrodes in the tibial epineurial and endoneurial spaces before and 30 minutes after epineurial devascularization. Normal epineurial oxygen tension was 40.4 ± (SD) 6.5 mm Hg before devascularization and 26.3 ± 12.3 mm Hg after (p <0.012). Normal endoneurial oxygen tension was 22.9 ± 6.0 mm Hg before devascularization and 14.3 ± 5.4 mm Hg after (p <0.003). The topography of nerve fiber injury in this experimental model is identical with the changes induced in the sciatic nerve by circumferential compression at 30 mm Hg which is also thought to impede epineurial circulation. This subperineurial pattern of demyelination and axonal degeneration is associated with experimental interference with the epineurial circulation and may be contrasted with the central fascicular degeneration caused by microsphere embolization of the vasa nervorum via the common iliac artery. The data suggest that ischemia is the mechanism for subperineurial fiber injury after epineurial devascularization and highlight the importance of the transperineurial vessels which connect the epineurial anastomotic circulation and endoneurial capillary network.

Original languageEnglish (US)
Pages (from-to)41-50
Number of pages10
JournalLaboratory Investigation
Volume65
Issue number1
StatePublished - 1991
Externally publishedYes

Fingerprint

Vasa Nervorum
Demyelinating Diseases
Blood Vessels
Oxygen
Ischemia
Iliac Artery
Wounds and Injuries
Microelectrodes
Sciatic Nerve
Platinum
Microspheres
Peripheral Nerves
Nerve Fibers
Sprague Dawley Rats
Electron Microscopy
Lasers
Theoretical Models

Keywords

  • Ischemia
  • Laser Doppler
  • Neuropathy

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Subperineurial demyelination associated with reduced nerve blood flow and oxygen tension after epineurial vascular stripping. / Myers, R. R.; Heckman, H. M.; Galbraith, James; Powell, H. C.

In: Laboratory Investigation, Vol. 65, No. 1, 1991, p. 41-50.

Research output: Contribution to journalArticle

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