Subcellular localization of the regulatory subunits of cyclic adenosine 3',5'-monophosphate-dependent protein kinase in bovine spermatozoa

S. Vijayaraghavan, G. E. Olson, S. NagDas, V. P. Winfrey, Daniel Carr

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Cyclic AMP (cAMP) is a regulator of sperm flagellar activity. The action of this cyclic nucleotide is presumably mediated by cAMP-dependent protein kinase (PKA). PKA is localized or targeted to specific subcellular sites through the interaction of PKA regulatory subunits with A-kinase anchoring proteins (AKAPs). We have recently shown that the addition of PKA anchoring inhibitor peptides to spermatozoa leads to the complete arrest of motility. A knowledge of the subcellular localization of PKA and AKAPs is essential for an understanding of how cAMP acts in spermatozoa. In this report, monospecific, affinity-purified, antipeptide antibodies were used to determine the distribution of the regulatory (R) subunit isoforms. Immunocytochemistry staining revealed that RIα and RIβ subunits are both localized predominantly in the acrosomal segment of the head, although they have distinct staining patterns within this region. In addition to the head, RIβ was observed in the midpiece of the tail while RIβ was detected in the connecting piece. RIIβ is prominent in the axonemal region of the flagellum but was not observed in the head region. These data suggest distinct roles for each of these isoforms in sperm functions such as motility and the acrosome reaction.

Original languageEnglish (US)
Pages (from-to)1517-1523
Number of pages7
JournalBiology of Reproduction
Volume57
Issue number6
StatePublished - 1997
Externally publishedYes

Fingerprint

Cyclic AMP
Protein Kinases
Spermatozoa
Head
Cyclic AMP-Dependent Protein Kinases
Protein Isoforms
Staining and Labeling
Acrosome Reaction
Flagella
Cyclic Nucleotides
Immunohistochemistry
Antibodies

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Embryology

Cite this

Subcellular localization of the regulatory subunits of cyclic adenosine 3',5'-monophosphate-dependent protein kinase in bovine spermatozoa. / Vijayaraghavan, S.; Olson, G. E.; NagDas, S.; Winfrey, V. P.; Carr, Daniel.

In: Biology of Reproduction, Vol. 57, No. 6, 1997, p. 1517-1523.

Research output: Contribution to journalArticle

@article{a63f11bda584475a942f4da279cfa401,
title = "Subcellular localization of the regulatory subunits of cyclic adenosine 3',5'-monophosphate-dependent protein kinase in bovine spermatozoa",
abstract = "Cyclic AMP (cAMP) is a regulator of sperm flagellar activity. The action of this cyclic nucleotide is presumably mediated by cAMP-dependent protein kinase (PKA). PKA is localized or targeted to specific subcellular sites through the interaction of PKA regulatory subunits with A-kinase anchoring proteins (AKAPs). We have recently shown that the addition of PKA anchoring inhibitor peptides to spermatozoa leads to the complete arrest of motility. A knowledge of the subcellular localization of PKA and AKAPs is essential for an understanding of how cAMP acts in spermatozoa. In this report, monospecific, affinity-purified, antipeptide antibodies were used to determine the distribution of the regulatory (R) subunit isoforms. Immunocytochemistry staining revealed that RIα and RIβ subunits are both localized predominantly in the acrosomal segment of the head, although they have distinct staining patterns within this region. In addition to the head, RIβ was observed in the midpiece of the tail while RIβ was detected in the connecting piece. RIIβ is prominent in the axonemal region of the flagellum but was not observed in the head region. These data suggest distinct roles for each of these isoforms in sperm functions such as motility and the acrosome reaction.",
author = "S. Vijayaraghavan and Olson, {G. E.} and S. NagDas and Winfrey, {V. P.} and Daniel Carr",
year = "1997",
language = "English (US)",
volume = "57",
pages = "1517--1523",
journal = "Biology of Reproduction",
issn = "0006-3363",
publisher = "Society for the Study of Reproduction",
number = "6",

}

TY - JOUR

T1 - Subcellular localization of the regulatory subunits of cyclic adenosine 3',5'-monophosphate-dependent protein kinase in bovine spermatozoa

AU - Vijayaraghavan, S.

AU - Olson, G. E.

AU - NagDas, S.

AU - Winfrey, V. P.

AU - Carr, Daniel

PY - 1997

Y1 - 1997

N2 - Cyclic AMP (cAMP) is a regulator of sperm flagellar activity. The action of this cyclic nucleotide is presumably mediated by cAMP-dependent protein kinase (PKA). PKA is localized or targeted to specific subcellular sites through the interaction of PKA regulatory subunits with A-kinase anchoring proteins (AKAPs). We have recently shown that the addition of PKA anchoring inhibitor peptides to spermatozoa leads to the complete arrest of motility. A knowledge of the subcellular localization of PKA and AKAPs is essential for an understanding of how cAMP acts in spermatozoa. In this report, monospecific, affinity-purified, antipeptide antibodies were used to determine the distribution of the regulatory (R) subunit isoforms. Immunocytochemistry staining revealed that RIα and RIβ subunits are both localized predominantly in the acrosomal segment of the head, although they have distinct staining patterns within this region. In addition to the head, RIβ was observed in the midpiece of the tail while RIβ was detected in the connecting piece. RIIβ is prominent in the axonemal region of the flagellum but was not observed in the head region. These data suggest distinct roles for each of these isoforms in sperm functions such as motility and the acrosome reaction.

AB - Cyclic AMP (cAMP) is a regulator of sperm flagellar activity. The action of this cyclic nucleotide is presumably mediated by cAMP-dependent protein kinase (PKA). PKA is localized or targeted to specific subcellular sites through the interaction of PKA regulatory subunits with A-kinase anchoring proteins (AKAPs). We have recently shown that the addition of PKA anchoring inhibitor peptides to spermatozoa leads to the complete arrest of motility. A knowledge of the subcellular localization of PKA and AKAPs is essential for an understanding of how cAMP acts in spermatozoa. In this report, monospecific, affinity-purified, antipeptide antibodies were used to determine the distribution of the regulatory (R) subunit isoforms. Immunocytochemistry staining revealed that RIα and RIβ subunits are both localized predominantly in the acrosomal segment of the head, although they have distinct staining patterns within this region. In addition to the head, RIβ was observed in the midpiece of the tail while RIβ was detected in the connecting piece. RIIβ is prominent in the axonemal region of the flagellum but was not observed in the head region. These data suggest distinct roles for each of these isoforms in sperm functions such as motility and the acrosome reaction.

UR - http://www.scopus.com/inward/record.url?scp=0030716780&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030716780&partnerID=8YFLogxK

M3 - Article

C2 - 9408263

AN - SCOPUS:0030716780

VL - 57

SP - 1517

EP - 1523

JO - Biology of Reproduction

JF - Biology of Reproduction

SN - 0006-3363

IS - 6

ER -