Subcellular Dynamics of Type II PKA in Neurons

Haining Zhong, Gek Ming Sia, Takashi R. Sato, Noah W. Gray, Tianyi Mao, Zaza Khuchua, Richard L. Huganir, Karel Svoboda

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

Protein kinase A (PKA) plays multiple roles in neurons. The localization and specificity of PKA are largely controlled by A-kinase anchoring proteins (AKAPs). However, the dynamics of PKA in neurons and the roles of specific AKAPs are poorly understood. We imaged the distribution of type II PKA in hippocampal and cortical layer 2/3 pyramidal neurons in vitro and in vivo. PKA was concentrated in dendritic shafts compared to the soma, axons, and dendritic spines. This spatial distribution was imposed by the microtubule-binding protein MAP2, indicating that MAP2 is the dominant AKAP in neurons. Following cAMP elevation, catalytic subunits dissociated from the MAP2-tethered regulatory subunits and rapidly became enriched in nearby spines. The spatial gradient of type II PKA between dendritic shafts and spines was critical for the regulation of synaptic strength and long-term potentiation. Therefore, the localization and activity-dependent translocation of type II PKA are important determinants of PKA function.

Original languageEnglish (US)
Pages (from-to)363-374
Number of pages12
JournalNeuron
Volume62
Issue number3
DOIs
StatePublished - May 14 2009
Externally publishedYes

Fingerprint

Cyclic AMP-Dependent Protein Kinase Type II
Cyclic AMP-Dependent Protein Kinases
Neurons
Protein Kinases
Dendritic Spines
Microtubule Proteins
Long-Term Potentiation
Pyramidal Cells
Carisoprodol
Axons
Catalytic Domain
Carrier Proteins
Spine

Keywords

  • MOLNEURO
  • SIGNALING

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Zhong, H., Sia, G. M., Sato, T. R., Gray, N. W., Mao, T., Khuchua, Z., ... Svoboda, K. (2009). Subcellular Dynamics of Type II PKA in Neurons. Neuron, 62(3), 363-374. https://doi.org/10.1016/j.neuron.2009.03.013

Subcellular Dynamics of Type II PKA in Neurons. / Zhong, Haining; Sia, Gek Ming; Sato, Takashi R.; Gray, Noah W.; Mao, Tianyi; Khuchua, Zaza; Huganir, Richard L.; Svoboda, Karel.

In: Neuron, Vol. 62, No. 3, 14.05.2009, p. 363-374.

Research output: Contribution to journalArticle

Zhong, H, Sia, GM, Sato, TR, Gray, NW, Mao, T, Khuchua, Z, Huganir, RL & Svoboda, K 2009, 'Subcellular Dynamics of Type II PKA in Neurons', Neuron, vol. 62, no. 3, pp. 363-374. https://doi.org/10.1016/j.neuron.2009.03.013
Zhong H, Sia GM, Sato TR, Gray NW, Mao T, Khuchua Z et al. Subcellular Dynamics of Type II PKA in Neurons. Neuron. 2009 May 14;62(3):363-374. https://doi.org/10.1016/j.neuron.2009.03.013
Zhong, Haining ; Sia, Gek Ming ; Sato, Takashi R. ; Gray, Noah W. ; Mao, Tianyi ; Khuchua, Zaza ; Huganir, Richard L. ; Svoboda, Karel. / Subcellular Dynamics of Type II PKA in Neurons. In: Neuron. 2009 ; Vol. 62, No. 3. pp. 363-374.
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