Subcellular distribution of rRNA and poly(A) RNA in hippocampal neurons in culture

Robin Kleiman, Gary Banker, Oswald Steward

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

In situ hybridization was used to assess the subcellular distribution of rRNA and poly(A) RNA in hippocampal neurons maintained in culture. Labeling produced with 35S-labeled probes to either rRNA or poly(A) was heaviest over the cell body with lighter, patchy labeling of proximal dendrites. In contrast, 3H-labeled probes labeled dendrites throughout their length, and the ratio of dendritic to cell body labeling was higher with 3H-labeled probes. There was no detectable labeling of axons of mature neurons with either probe. The pattern of hybridization produced by 35S-labeled oligonucleotide probes to rRNA varied depending on the concentration of the oligonucleotide. These studies provide the first detailed study of the subcellular distribution of rRNA and poly(A) RNA in neurons, and highlight technical issues to consider when evaluating results of hybridizations carried out with 35S- and 3H-labeled probes on cells in culture.

Original languageEnglish (US)
Pages (from-to)305-312
Number of pages8
JournalMolecular Brain Research
Volume20
Issue number4
DOIs
StatePublished - Dec 1993

Keywords

  • Autoradiography
  • Dendrite
  • In situ hybridization
  • Poly(A) RNA
  • rRNA

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience

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