Subcellular distribution of rRNA and poly(A) RNA in hippocampal neurons in culture

Robin Kleiman, Gary Banker, Oswald Steward

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

In situ hybridization was used to assess the subcellular distribution of rRNA and poly(A) RNA in hippocampal neurons maintained in culture. Labeling produced with 35S-labeled probes to either rRNA or poly(A) was heaviest over the cell body with lighter, patchy labeling of proximal dendrites. In contrast, 3H-labeled probes labeled dendrites throughout their length, and the ratio of dendritic to cell body labeling was higher with 3H-labeled probes. There was no detectable labeling of axons of mature neurons with either probe. The pattern of hybridization produced by 35S-labeled oligonucleotide probes to rRNA varied depending on the concentration of the oligonucleotide. These studies provide the first detailed study of the subcellular distribution of rRNA and poly(A) RNA in neurons, and highlight technical issues to consider when evaluating results of hybridizations carried out with 35S- and 3H-labeled probes on cells in culture.

Original languageEnglish (US)
Pages (from-to)305-312
Number of pages8
JournalMolecular Brain Research
Volume20
Issue number4
DOIs
StatePublished - 1993
Externally publishedYes

Fingerprint

Dendrites
Neurons
Messenger RNA
Oligonucleotide Probes
Oligonucleotides
Dendritic Cells
In Situ Hybridization
Axons
Cell Culture Techniques
Cell Body
poly(A) ribosomal RNA

Keywords

  • Autoradiography
  • Dendrite
  • In situ hybridization
  • Poly(A) RNA
  • rRNA

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience

Cite this

Subcellular distribution of rRNA and poly(A) RNA in hippocampal neurons in culture. / Kleiman, Robin; Banker, Gary; Steward, Oswald.

In: Molecular Brain Research, Vol. 20, No. 4, 1993, p. 305-312.

Research output: Contribution to journalArticle

Kleiman, Robin ; Banker, Gary ; Steward, Oswald. / Subcellular distribution of rRNA and poly(A) RNA in hippocampal neurons in culture. In: Molecular Brain Research. 1993 ; Vol. 20, No. 4. pp. 305-312.
@article{e090459841344e70a699b7c6d7dd6eda,
title = "Subcellular distribution of rRNA and poly(A) RNA in hippocampal neurons in culture",
abstract = "In situ hybridization was used to assess the subcellular distribution of rRNA and poly(A) RNA in hippocampal neurons maintained in culture. Labeling produced with 35S-labeled probes to either rRNA or poly(A) was heaviest over the cell body with lighter, patchy labeling of proximal dendrites. In contrast, 3H-labeled probes labeled dendrites throughout their length, and the ratio of dendritic to cell body labeling was higher with 3H-labeled probes. There was no detectable labeling of axons of mature neurons with either probe. The pattern of hybridization produced by 35S-labeled oligonucleotide probes to rRNA varied depending on the concentration of the oligonucleotide. These studies provide the first detailed study of the subcellular distribution of rRNA and poly(A) RNA in neurons, and highlight technical issues to consider when evaluating results of hybridizations carried out with 35S- and 3H-labeled probes on cells in culture.",
keywords = "Autoradiography, Dendrite, In situ hybridization, Poly(A) RNA, rRNA",
author = "Robin Kleiman and Gary Banker and Oswald Steward",
year = "1993",
doi = "10.1016/0169-328X(93)90057-V",
language = "English (US)",
volume = "20",
pages = "305--312",
journal = "Brain Research",
issn = "0006-8993",
publisher = "Elsevier",
number = "4",

}

TY - JOUR

T1 - Subcellular distribution of rRNA and poly(A) RNA in hippocampal neurons in culture

AU - Kleiman, Robin

AU - Banker, Gary

AU - Steward, Oswald

PY - 1993

Y1 - 1993

N2 - In situ hybridization was used to assess the subcellular distribution of rRNA and poly(A) RNA in hippocampal neurons maintained in culture. Labeling produced with 35S-labeled probes to either rRNA or poly(A) was heaviest over the cell body with lighter, patchy labeling of proximal dendrites. In contrast, 3H-labeled probes labeled dendrites throughout their length, and the ratio of dendritic to cell body labeling was higher with 3H-labeled probes. There was no detectable labeling of axons of mature neurons with either probe. The pattern of hybridization produced by 35S-labeled oligonucleotide probes to rRNA varied depending on the concentration of the oligonucleotide. These studies provide the first detailed study of the subcellular distribution of rRNA and poly(A) RNA in neurons, and highlight technical issues to consider when evaluating results of hybridizations carried out with 35S- and 3H-labeled probes on cells in culture.

AB - In situ hybridization was used to assess the subcellular distribution of rRNA and poly(A) RNA in hippocampal neurons maintained in culture. Labeling produced with 35S-labeled probes to either rRNA or poly(A) was heaviest over the cell body with lighter, patchy labeling of proximal dendrites. In contrast, 3H-labeled probes labeled dendrites throughout their length, and the ratio of dendritic to cell body labeling was higher with 3H-labeled probes. There was no detectable labeling of axons of mature neurons with either probe. The pattern of hybridization produced by 35S-labeled oligonucleotide probes to rRNA varied depending on the concentration of the oligonucleotide. These studies provide the first detailed study of the subcellular distribution of rRNA and poly(A) RNA in neurons, and highlight technical issues to consider when evaluating results of hybridizations carried out with 35S- and 3H-labeled probes on cells in culture.

KW - Autoradiography

KW - Dendrite

KW - In situ hybridization

KW - Poly(A) RNA

KW - rRNA

UR - http://www.scopus.com/inward/record.url?scp=0027435703&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027435703&partnerID=8YFLogxK

U2 - 10.1016/0169-328X(93)90057-V

DO - 10.1016/0169-328X(93)90057-V

M3 - Article

C2 - 7906850

AN - SCOPUS:0027435703

VL - 20

SP - 305

EP - 312

JO - Brain Research

JF - Brain Research

SN - 0006-8993

IS - 4

ER -