Subcellular distribution of protein kinase C isozymes during cardioplegic arrest

Źivojin S. Jonjev, Dorie W. Schwertz, Jennifer M. Beck, James Ross, William R. Law

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Background: On the basis of the hypothesis that cardioplegia-associated myocardial depression was due to activation of protein kinase C, we examined whether specific protein kinase C isozymes would translocate to a cellular fraction containing myofilaments. Methods: Isolated rat hearts were perfused with Krebs-Ringer bicarbonate buffer for 30 minutes and arrested with 4°C St Thomas No. 2 cardioplegic solution for 0 to 120 minutes (n = 5 per group). The 3 fractions of the left ventricle tissue represented the myofibrillar/nuclear fraction (P1), membranes (P2), and cytosol (supernatant). The distributions of protein kinase C isozymes α, δ, ε, and η were examined after separation by electrophoresis, immunoblotting/chemiluminescence, and densitometry. Results: A significant increase in protein kinase C-δ in the P1 fraction was detected after 5 minutes of cardioplegic arrest and remained increased for 60 minutes. Increases in P1 protein kinase C-α and -ε were seen transiently at 5 minutes, and protein kinase C-ε demonstrated a secondary increase in P1 at 30 to 60 minutes. There was also a significant relative increase in protein kinase C-α and protein kinase C-δ in the P2 fraction after 60 minutes of cardioplegia. Conclusions: These data are consistent with our hypothesis that activation of protein kinase C isozymes is associated with altered myofilament function after cardioplegic arrest.

Original languageEnglish (US)
Pages (from-to)1880-1885
Number of pages6
JournalJournal of Thoracic and Cardiovascular Surgery
Volume126
Issue number6
DOIs
StatePublished - Dec 2003
Externally publishedYes

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Protein Kinase C
Isoenzymes
Induced Heart Arrest
Myofibrils
Cardioplegic Solutions
Densitometry
Bicarbonates
Luminescence
Immunoblotting
Cytosol
Heart Ventricles
Electrophoresis
Buffers
Membranes

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Surgery

Cite this

Subcellular distribution of protein kinase C isozymes during cardioplegic arrest. / Jonjev, Źivojin S.; Schwertz, Dorie W.; Beck, Jennifer M.; Ross, James; Law, William R.

In: Journal of Thoracic and Cardiovascular Surgery, Vol. 126, No. 6, 12.2003, p. 1880-1885.

Research output: Contribution to journalArticle

Jonjev, Źivojin S. ; Schwertz, Dorie W. ; Beck, Jennifer M. ; Ross, James ; Law, William R. / Subcellular distribution of protein kinase C isozymes during cardioplegic arrest. In: Journal of Thoracic and Cardiovascular Surgery. 2003 ; Vol. 126, No. 6. pp. 1880-1885.
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