Subcellular differentiation in sporulating yeast cells

Stephen Kurtz, Susan Lindquist

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

We have previously described the induction of two sets of sporulation-specific mRNAs in Saccharomyces cerevisiae. Herein we correlate the appearance of these RNAs with the major morphogenic events of sporulation, and we analyze the spatial distribution of the RNAs within the ascus. Several observations suggest that the first set of messages is involved in spore wall synthesis. In fractionation experiments, these mRNAs are detected in the ascal cytoplasm but not in developing spores, indicating that the proteinaceous component of the spore wall is synthesized from the external compartment. The second set of messages is induced later in the course of spore maturation. These mRNAs accumulate within the spores and, unlike the first set of mRNAs, are retained in mature asci until the early stages of germination. We conclude that the development of ascospores proceeds through the differentiation of functionally distinct subcellular compartments.

Original languageEnglish (US)
Pages (from-to)771-779
Number of pages9
JournalCell
Volume45
Issue number5
DOIs
StatePublished - Jun 6 1986
Externally publishedYes

Fingerprint

Spores
Yeast
Yeasts
Cells
Messenger RNA
RNA
Fractionation
Spatial Analysis
Spatial distribution
Germination
Saccharomyces cerevisiae
Cytoplasm
Experiments

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Subcellular differentiation in sporulating yeast cells. / Kurtz, Stephen; Lindquist, Susan.

In: Cell, Vol. 45, No. 5, 06.06.1986, p. 771-779.

Research output: Contribution to journalArticle

Kurtz, Stephen ; Lindquist, Susan. / Subcellular differentiation in sporulating yeast cells. In: Cell. 1986 ; Vol. 45, No. 5. pp. 771-779.
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