TY - JOUR
T1 - Subcellular differentiation in sporulating yeast cells
AU - Kurtz, Stephen
AU - Lindquist, Susan
N1 - Funding Information:
We are grateful to H. Swift, E. Sirkin, and E. Gordon for advice and critical comments; to M. Welsh for generously providing purified SRPs and micmsomal membranes; to J. Segall, A. Percival-Smith, T Garber, and F. Rosenberg for DNA probes and hybrid selection data; to Sagami Paul for expert technical assistance, and to Mary Haynes and Sharlene Johnson for preparing the manuscript. This work was supported by National Institutes of Health grant GM 35462, with facilities supported by National Cancer Institute grant CA 44599.
PY - 1986/6/6
Y1 - 1986/6/6
N2 - We have previously described the induction of two sets of sporulation-specific mRNAs in Saccharomyces cerevisiae. Herein we correlate the appearance of these RNAs with the major morphogenic events of sporulation, and we analyze the spatial distribution of the RNAs within the ascus. Several observations suggest that the first set of messages is involved in spore wall synthesis. In fractionation experiments, these mRNAs are detected in the ascal cytoplasm but not in developing spores, indicating that the proteinaceous component of the spore wall is synthesized from the external compartment. The second set of messages is induced later in the course of spore maturation. These mRNAs accumulate within the spores and, unlike the first set of mRNAs, are retained in mature asci until the early stages of germination. We conclude that the development of ascospores proceeds through the differentiation of functionally distinct subcellular compartments.
AB - We have previously described the induction of two sets of sporulation-specific mRNAs in Saccharomyces cerevisiae. Herein we correlate the appearance of these RNAs with the major morphogenic events of sporulation, and we analyze the spatial distribution of the RNAs within the ascus. Several observations suggest that the first set of messages is involved in spore wall synthesis. In fractionation experiments, these mRNAs are detected in the ascal cytoplasm but not in developing spores, indicating that the proteinaceous component of the spore wall is synthesized from the external compartment. The second set of messages is induced later in the course of spore maturation. These mRNAs accumulate within the spores and, unlike the first set of mRNAs, are retained in mature asci until the early stages of germination. We conclude that the development of ascospores proceeds through the differentiation of functionally distinct subcellular compartments.
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U2 - 10.1016/0092-8674(86)90791-9
DO - 10.1016/0092-8674(86)90791-9
M3 - Article
C2 - 3518948
AN - SCOPUS:0023043285
SN - 0092-8674
VL - 45
SP - 771
EP - 779
JO - Cell
JF - Cell
IS - 5
ER -