STX, a novel nonsteroidal estrogenic compound, induces rapid action in primate GnRH neuronal calcium dynamics and peptide release

B. P. Kenealy, K. L. Keen, Oline Ronnekleiv, Ei Terasawa

Research output: Contribution to journalArticle

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Abstract

Previously, we reported that 1 nM 17β-estradiol (E2) induces a rapid action, which is, in part, mediated through the G protein-coupled receptor GPR30 in primate GnRH neurons. Because it has been reported that the diphenylacrylamide compound, STX, causes estrogenic action in the mouse and guinea pig hypothalamus, the present study examined effects of STX in primate GnRH neurons and whether there is an action independent of GPR30. Results are summarized as follows. STX (10 nM) exposure increased 1) the oscillation frequency of intracellular calcium concentration ([Ca 2+]i), 2) the percentage of cells stimulated, and 3) the synchronization frequency of [Ca2+]i oscillations. STX (10-100 nM) also stimulated GnRH release. The effects of STX on both [Ca 2+]i oscillations and GnRH release were similar to those caused by E2 (1 nM), although with less magnitude. STX (10 nM)-induced changes in [Ca2+]i oscillations were not altered by GPR30 small interfering RNA transfection, indicating that STX-sensitive receptors differ from GPR30. Finally, a higher dose of E 2 (10 nM) induced a larger change in [Ca2+]i oscillations than that with a smaller dose of E2 (1 nM), and the effects of 10 nM E2 were reduced but not completely blocked by GPR30 small interfering RNA transfection, indicating that the effects of 10 nM E 2 in primate GnRH neurons are mediated by multiple membrane receptors, including GPR30 and STX-sensitive receptors. Collectively, the rapid action of E2 mediated through GPR30 differs from that mediated through STX-sensitive receptors. The molecular structure of the STX-sensitive receptor remains to be identified.

Original languageEnglish (US)
Pages (from-to)3182-3191
Number of pages10
JournalEndocrinology
Volume152
Issue number8
DOIs
StatePublished - Aug 2011

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Gonadotropin-Releasing Hormone
Primates
Estrogens
Calcium
Peptides
Neurons
Small Interfering RNA
Transfection
G-Protein-Coupled Receptors
Molecular Structure
Hypothalamus
Estradiol
Guinea Pigs
Membranes

ASJC Scopus subject areas

  • Endocrinology

Cite this

STX, a novel nonsteroidal estrogenic compound, induces rapid action in primate GnRH neuronal calcium dynamics and peptide release. / Kenealy, B. P.; Keen, K. L.; Ronnekleiv, Oline; Terasawa, Ei.

In: Endocrinology, Vol. 152, No. 8, 08.2011, p. 3182-3191.

Research output: Contribution to journalArticle

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