Study of the structural dynamics of the E. coli 70S ribosome using real-space refinement

Haixiao Gao; Jayati Sengupta; Mikel Valle; Andrei Korostelev; Narayanan Eswar; Scott M. Stagg; Patrick Van Roey; Rajendra K. Agrawal; Stephen C. Harvey; Andrej Sali; Michael S. Chapman; Joachim Frank

doi:10.1016/S0092-8674(03)00427-6

Study of the structural dynamics of the E. coli 70S ribosome using real-space refinement

Haixiao Gao, Jayati Sengupta, Mikel Valle, Andrei Korostelev, Narayanan Eswar, Scott M. Stagg, Patrick Van Roey, Rajendra K. Agrawal, Stephen C. Harvey, Andrej Sali, Michael S. Chapman, Joachim Frank

Research output: Contribution to journal › Article › peer-review

249 Scopus citations

Abstract

Cryo-EM density maps showing the 70S ribosome of E. coli in two different functional states related by a ratchet-like motion were analyzed using real-space refinement. Comparison of the two resulting atomic models shows that the ribosome changes from a compact structure to a looser one, coupled with the rearrangement of many of the proteins. Furthermore, in contrast to the unchanged inter-subunit bridges formed wholly by RNA, the bridges involving proteins undergo large conformational changes following the ratchet-like motion, suggesting an important role of ribosomal proteins in facilitating the dynamics of translation.

Original language	English (US)
Pages (from-to)	789-801
Number of pages	13
Journal	Cell
Volume	113
Issue number	6
DOIs	https://doi.org/10.1016/S0092-8674(03)00427-6
State	Published - Jun 13 2003
Externally published	Yes

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)

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10.1016/S0092-8674(03)00427-6

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@article{a2f0f14341ea4229903799039abc418a,

title = "Study of the structural dynamics of the E. coli 70S ribosome using real-space refinement",

abstract = "Cryo-EM density maps showing the 70S ribosome of E. coli in two different functional states related by a ratchet-like motion were analyzed using real-space refinement. Comparison of the two resulting atomic models shows that the ribosome changes from a compact structure to a looser one, coupled with the rearrangement of many of the proteins. Furthermore, in contrast to the unchanged inter-subunit bridges formed wholly by RNA, the bridges involving proteins undergo large conformational changes following the ratchet-like motion, suggesting an important role of ribosomal proteins in facilitating the dynamics of translation.",

author = "Haixiao Gao and Jayati Sengupta and Mikel Valle and Andrei Korostelev and Narayanan Eswar and Stagg, {Scott M.} and {Van Roey}, Patrick and Agrawal, {Rajendra K.} and Harvey, {Stephen C.} and Andrej Sali and Chapman, {Michael S.} and Joachim Frank",

note = "Funding Information: We thank Michael Watters and Yu Chen for preparing the illustrations. This work was supported by NIH R37 GM 29169 (J.F.), NIH R01 GM 55440 (J.F.), NIH P01 GM 64676 (T.A. Cross/MSC), NSF DBI-98-08098 (M.S.C.), NIH R01 GM 54762 (A.S.), NIH P50 GM62529, Sun Academic Equipment Grant. EDUD-7824-020257-US (A.S.), NIH R01 GM 53827(SCH), and NIH R01 GM 61576 (R.K.A.). ",

year = "2003",

month = jun,

day = "13",

doi = "10.1016/S0092-8674(03)00427-6",

language = "English (US)",

volume = "113",

pages = "789--801",

journal = "Cell",

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TY - JOUR

T1 - Study of the structural dynamics of the E. coli 70S ribosome using real-space refinement

AU - Gao, Haixiao

AU - Sengupta, Jayati

AU - Valle, Mikel

AU - Korostelev, Andrei

AU - Eswar, Narayanan

AU - Stagg, Scott M.

AU - Van Roey, Patrick

AU - Agrawal, Rajendra K.

AU - Harvey, Stephen C.

AU - Sali, Andrej

AU - Chapman, Michael S.

AU - Frank, Joachim

N1 - Funding Information: We thank Michael Watters and Yu Chen for preparing the illustrations. This work was supported by NIH R37 GM 29169 (J.F.), NIH R01 GM 55440 (J.F.), NIH P01 GM 64676 (T.A. Cross/MSC), NSF DBI-98-08098 (M.S.C.), NIH R01 GM 54762 (A.S.), NIH P50 GM62529, Sun Academic Equipment Grant. EDUD-7824-020257-US (A.S.), NIH R01 GM 53827(SCH), and NIH R01 GM 61576 (R.K.A.).

PY - 2003/6/13

Y1 - 2003/6/13

N2 - Cryo-EM density maps showing the 70S ribosome of E. coli in two different functional states related by a ratchet-like motion were analyzed using real-space refinement. Comparison of the two resulting atomic models shows that the ribosome changes from a compact structure to a looser one, coupled with the rearrangement of many of the proteins. Furthermore, in contrast to the unchanged inter-subunit bridges formed wholly by RNA, the bridges involving proteins undergo large conformational changes following the ratchet-like motion, suggesting an important role of ribosomal proteins in facilitating the dynamics of translation.

AB - Cryo-EM density maps showing the 70S ribosome of E. coli in two different functional states related by a ratchet-like motion were analyzed using real-space refinement. Comparison of the two resulting atomic models shows that the ribosome changes from a compact structure to a looser one, coupled with the rearrangement of many of the proteins. Furthermore, in contrast to the unchanged inter-subunit bridges formed wholly by RNA, the bridges involving proteins undergo large conformational changes following the ratchet-like motion, suggesting an important role of ribosomal proteins in facilitating the dynamics of translation.

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DO - 10.1016/S0092-8674(03)00427-6

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C2 - 12809609

AN - SCOPUS:0038275890

SN - 0092-8674

VL - 113

SP - 789

EP - 801

JO - Cell

JF - Cell

IS - 6

ER -

Study of the structural dynamics of the E. coli 70S ribosome using real-space refinement

Abstract

ASJC Scopus subject areas

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