Study of the promoter regions of human mitochondria!- Glycerol-3-phosphate dehydrogenase

Qiuming Gong, L. J. Brown, S. R. Statten, M. J. MacDonald

Research output: Contribution to journalArticle

Abstract

Mitochondrial glycerol-3-phosphate dehydrogenase (mGPD), the key enzyme of the glycerol-phosphate shuttle, is encoded in the nucleus and is located in the outer surface of the inner mitochondrial membrane. mGPD is abundant in the normal pancreatic islet. The glycerol phosphate shuttle is believed to play a role in the metabolic signaling of insulin secretion in islet beta cells, and the activity of mGPD is low in several rodent models of non-insulin dependent diabetes. Our analyses of the 5'-untranslated region of the human mGPD mRNA and its genomic organization revealed two alternative first exons designated E1A and E1B. Each of these exons is alternatively spliced to a common exon (exon 2). Sequencing of the 5'flanking regions containing the two putative mGPD promoters PE1A and PE1B indicated that they contain no TATA box, but have several binding sites for transcription factors Sp1 and AP-2. We assessed promoter activity in transient expression assays using luciferase as a reporter. Luciferase activity of PE1A was 10% of PE1B in both INS-1 and HeLa cells. Progressive 5' deletions in the region immediately proximal to E1B resulted in sequentially larger decreases in promoter activity. These findings suggest that the human mGPD gene contains at least two promoters that control transcription and provide a molecular basis for the alternative splicing of mGPD transcripts.

Original languageEnglish (US)
JournalFASEB Journal
Volume11
Issue number9
StatePublished - 1997
Externally publishedYes

Fingerprint

glycerol-3-phosphate dehydrogenase
Glycerolphosphate Dehydrogenase
Mitochondria
Genetic Promoter Regions
mitochondria
promoter regions
exons
Exons
alternative splicing
luciferase
Luciferases
Islets of Langerhans
Glycerol
glycerol
Transcription Factor AP-2
Phosphates
phosphates
Sp1 Transcription Factor
TATA box
TATA Box

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Study of the promoter regions of human mitochondria!- Glycerol-3-phosphate dehydrogenase. / Gong, Qiuming; Brown, L. J.; Statten, S. R.; MacDonald, M. J.

In: FASEB Journal, Vol. 11, No. 9, 1997.

Research output: Contribution to journalArticle

Gong, Qiuming ; Brown, L. J. ; Statten, S. R. ; MacDonald, M. J. / Study of the promoter regions of human mitochondria!- Glycerol-3-phosphate dehydrogenase. In: FASEB Journal. 1997 ; Vol. 11, No. 9.
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