Studies on the ionic mechanism for the neuromodulatory actions of adenosine in the brain

Mary L. Michaelis, Karl K. Johe, Bita Moghaddam, Ralph N. Adams

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Although much evidence exists to support the modulation of neurotransmitter release by adenosine in both the central and peripheral nervous systems, the ionic mechanisms involved in this process are still not established. In our initial series,of studies, the effects of adenosine analogs on depolarization-induced 45Ca influx into synaptosomes were examined, but no reduction in 45Ca uptake was observed in the presence of these agents, regardless of whether synaptosomes were depolarized by KCl or veratridine. The possibility that synaptosomal adenosine receptors are coupled directly to K+ channels and that an increase in K+ conductance is the primary ionic event initiated by adenosine was examined in resealed synaptic plasma membranes. We followed the movement of K+ through the use of the labeled lipophilic anion [35S]thiocyanate ([35S]SCN-), which distributes across membranes according to the existing membrane potential. Both 2-chloroadenosine and adenosine-5′-cyclopropyl carboxamide produced dose-dependent increases in SCN- uptake, indicating enhanced K+ fluxes across the synaptic membranes. These effects of adenosine receptor agonists on membrane K+ conductance were blocked by receptor antagonists such as isobutylmethylxanthine. In addition, the placement of a K+-selective microelectrode in the caudate nuclei of intact, anesthetized rats revealed a substantial increase in the extracellular K+ concentration when adenosine was released onto the cells from a pipet in the assembly containing the ion-selective microelectrode. The results of these studies at both the membrane level and in the intact brain suggest that the initial event in the neuromodulatory actions of adenosine is an increase in the membrane conductance for K+ rather than inhibition of the voltage sensitive Ca2+ channels.

Original languageEnglish (US)
Pages (from-to)249-260
Number of pages12
JournalBrain Research
Volume473
Issue number2
DOIs
StatePublished - Nov 15 1988
Externally publishedYes

Fingerprint

Adenosine
Brain
Synaptic Membranes
Membranes
Synaptosomes
Microelectrodes
2-Chloroadenosine
Purinergic P1 Receptor Agonists
Veratridine
Purinergic P1 Receptors
Caudate Nucleus
Peripheral Nervous System
Membrane Potentials
Anions
Neurotransmitter Agents
Central Nervous System
Cell Membrane
Ions

Keywords

  • Adenosine
  • Ion-selective microelectrode
  • Ionic mechanism
  • K-conductance
  • Lipophilic ions
  • Neuromodulation
  • Synaptosome

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Studies on the ionic mechanism for the neuromodulatory actions of adenosine in the brain. / Michaelis, Mary L.; Johe, Karl K.; Moghaddam, Bita; Adams, Ralph N.

In: Brain Research, Vol. 473, No. 2, 15.11.1988, p. 249-260.

Research output: Contribution to journalArticle

Michaelis, Mary L. ; Johe, Karl K. ; Moghaddam, Bita ; Adams, Ralph N. / Studies on the ionic mechanism for the neuromodulatory actions of adenosine in the brain. In: Brain Research. 1988 ; Vol. 473, No. 2. pp. 249-260.
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