Studies on the control of myelinogenesis. IV. Neuronal induction of Schwann cell myelin-specific protein synthesis during nerve fiber regeneration

M. J. Politis, N. Sternberger, K. Ederle, Peter Spencer

Research output: Contribution to journalArticle

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Abstract

The temporal sequence of axon-Schwann cell interaction during regeneration is examined in cat tibial nerves surgically denervated for 8 weeks, and subsequently, coapted to a freshly severed peroneal nerve for 3 weeks. Prior to association with regenerating axons. Schwann cells resident in denervated and reinnervated stumps failed to synthesize proteins co-migrant with P0, P1, and P2 synthesis of amino acid-labeled protein co-migrating with myelin-specific proteins prior to elaboration of myelin lamellae. Radioactivity from these peaks was precipitated by antibodies raised against myelin-specific proteins. The synthesis of P1 and P2 proteins was evident before P0 synthesis in reinnervated stumps. Immunocytochemical staining with antibody to P0, P1, P2 and myelinassociated glycoprotein (MAG) appeared only after myelin lamellae had been formed. These data suggest that Schwann cells; synthesize protein co-migrant with P1, P2, P0, and MAG in normal cat nerves; cease detectable manufacture of these proteins after axonal loss; regain their capacity to synthesize these proteins upon re-establishment of axonal association; and during regeneration, express the synthesis of P1 and P2 before that of P0.

Original languageEnglish (US)
Pages (from-to)1252-1266
Number of pages15
JournalJournal of Neuroscience
Volume2
Issue number9
StatePublished - 1982
Externally publishedYes

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Myelin Proteins
Nerve Regeneration
Schwann Cells
Nerve Fibers
Proteins
Myelin Sheath
Axons
Regeneration
Cats
Myelin P0 Protein
Tibial Nerve
Peroneal Nerve
Antibodies
Cell Communication
Radioactivity
Glycoproteins
Staining and Labeling
Amino Acids

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Studies on the control of myelinogenesis. IV. Neuronal induction of Schwann cell myelin-specific protein synthesis during nerve fiber regeneration. / Politis, M. J.; Sternberger, N.; Ederle, K.; Spencer, Peter.

In: Journal of Neuroscience, Vol. 2, No. 9, 1982, p. 1252-1266.

Research output: Contribution to journalArticle

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AB - The temporal sequence of axon-Schwann cell interaction during regeneration is examined in cat tibial nerves surgically denervated for 8 weeks, and subsequently, coapted to a freshly severed peroneal nerve for 3 weeks. Prior to association with regenerating axons. Schwann cells resident in denervated and reinnervated stumps failed to synthesize proteins co-migrant with P0, P1, and P2 synthesis of amino acid-labeled protein co-migrating with myelin-specific proteins prior to elaboration of myelin lamellae. Radioactivity from these peaks was precipitated by antibodies raised against myelin-specific proteins. The synthesis of P1 and P2 proteins was evident before P0 synthesis in reinnervated stumps. Immunocytochemical staining with antibody to P0, P1, P2 and myelinassociated glycoprotein (MAG) appeared only after myelin lamellae had been formed. These data suggest that Schwann cells; synthesize protein co-migrant with P1, P2, P0, and MAG in normal cat nerves; cease detectable manufacture of these proteins after axonal loss; regain their capacity to synthesize these proteins upon re-establishment of axonal association; and during regeneration, express the synthesis of P1 and P2 before that of P0.

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