Studies of solubilized sarcoplasmic reticulum

B. H. McFarland; G. Inesi

doi:10.1016/0006-291X(70)90494-8

Studies of solubilized sarcoplasmic reticulum

B. H. McFarland, G. Inesi

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

The non-ionic detergent Triton X-100 solubilizes most of the protein and phospholipid from the sarcoplasmic reticulum of rabbit skeletal muscle. The calcium dependent ATPase of the sarcoplasmic reticulum is found in the soluble fraction whereas the basic ATPase activity remains insoluble in Triton X-100. Analytical ultracentrifugation and disc electrophoresis indicate that the solubilized material consists of particles having a molecular weight of approximately 80,000 daltons. These particles have a strong tendency to aggregate.

Original language	English (US)
Pages (from-to)	239-243
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	41
Issue number	1
DOIs	https://doi.org/10.1016/0006-291X(70)90494-8
State	Published - Oct 9 1970
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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abstract = "The non-ionic detergent Triton X-100 solubilizes most of the protein and phospholipid from the sarcoplasmic reticulum of rabbit skeletal muscle. The calcium dependent ATPase of the sarcoplasmic reticulum is found in the soluble fraction whereas the basic ATPase activity remains insoluble in Triton X-100. Analytical ultracentrifugation and disc electrophoresis indicate that the solubilized material consists of particles having a molecular weight of approximately 80,000 daltons. These particles have a strong tendency to aggregate.",

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AU - Inesi, G.

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AB - The non-ionic detergent Triton X-100 solubilizes most of the protein and phospholipid from the sarcoplasmic reticulum of rabbit skeletal muscle. The calcium dependent ATPase of the sarcoplasmic reticulum is found in the soluble fraction whereas the basic ATPase activity remains insoluble in Triton X-100. Analytical ultracentrifugation and disc electrophoresis indicate that the solubilized material consists of particles having a molecular weight of approximately 80,000 daltons. These particles have a strong tendency to aggregate.

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Studies of solubilized sarcoplasmic reticulum

Abstract

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