Studies of ion channels using expressed protein ligation

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10 Scopus citations

Abstract

Expressed protein ligation (EPL) is a semisynthetic technique for the chemoselective ligation of a synthetic peptide to a recombinant peptide that results in a native peptide bond at the ligation site. EPL therefore allows us to engineer proteins with chemically defined, site-specific modifications. While EPL has been used mainly in investigations of soluble proteins, in recent years it has been increasingly used in investigations of integral membrane proteins. These include studies on the KcsA K+ channel, the non-selective cation channel NaK, and the porin OmpF. These studies are discussed in this review.

Original languageEnglish (US)
Pages (from-to)797-802
Number of pages6
JournalCurrent Opinion in Chemical Biology
Volume14
Issue number6
DOIs
StatePublished - Dec 1 2010

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

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