TY - JOUR
T1 - Studies of ion channels using expressed protein ligation
AU - Focke, Paul J.
AU - Valiyaveetil, Francis I.
N1 - Funding Information:
Research in the Valiyaveetil laboratory is supported by National Institutes of Health ( GM087546 ), and a Scientist Development Grant from the American Heart Association ( 0835166N ). FIV is a Pew Scholar in the Biomedical Sciences.
PY - 2010/12
Y1 - 2010/12
N2 - Expressed protein ligation (EPL) is a semisynthetic technique for the chemoselective ligation of a synthetic peptide to a recombinant peptide that results in a native peptide bond at the ligation site. EPL therefore allows us to engineer proteins with chemically defined, site-specific modifications. While EPL has been used mainly in investigations of soluble proteins, in recent years it has been increasingly used in investigations of integral membrane proteins. These include studies on the KcsA K+ channel, the non-selective cation channel NaK, and the porin OmpF. These studies are discussed in this review.
AB - Expressed protein ligation (EPL) is a semisynthetic technique for the chemoselective ligation of a synthetic peptide to a recombinant peptide that results in a native peptide bond at the ligation site. EPL therefore allows us to engineer proteins with chemically defined, site-specific modifications. While EPL has been used mainly in investigations of soluble proteins, in recent years it has been increasingly used in investigations of integral membrane proteins. These include studies on the KcsA K+ channel, the non-selective cation channel NaK, and the porin OmpF. These studies are discussed in this review.
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U2 - 10.1016/j.cbpa.2010.09.014
DO - 10.1016/j.cbpa.2010.09.014
M3 - Article
C2 - 20965773
AN - SCOPUS:78649827915
SN - 1367-5931
VL - 14
SP - 797
EP - 802
JO - Current Opinion in Chemical Biology
JF - Current Opinion in Chemical Biology
IS - 6
ER -