@article{21c6ed2ba9c24c308f3731220be3aee6,
title = "Studies in organ preservation by actual freezing and reduction of the freezing point",
abstract = "Methods of organ preservation under study at the University of Oregon Medical School have been briefly reviewed. It is possible to preserve organs in a viable state by actually depressing the freezing point. The best long-term method of preservation will probably be actual freezing. This important field, cryobiology, is just in its infancy and yet possible applications are immense. In this report, the primary pharmacological actions of dimethyl sulfoxide previously published have been listed.",
author = "Jacob, {Stanley W.}",
note = "Funding Information: The problems involved in the realm of whole organ preservation by freezing have been investigated by the author for several years. Initially, studies were undertaken on the resistance of isolated tissues, particularly human, to temperatures approaching the absolute zero. Human conjunctival cells saturated with 6% glycerol, human spermatozoa without glycerol, human spermatozoa pretreated with one part glycerol to nine parts semen, the Coxsackie virus without glycerol, and dog and rabbit skin previously immersed in 15% glycerol-saline were frozen to -2722°C. Aft.er this temperature had * Presented at the First Annual Meeting, Society for Cryobiology, August 24-26, 1964, Washington, D. C. {\textquoteleft}i Supported in part by Grants CA04780-05Sl and CA-7221 from the National Cancer Institute. National Institutes of Health. $ Markle Scholar in Medical Sciences.",
year = "1964",
doi = "10.1016/0011-2240(64)90009-4",
language = "English (US)",
volume = "1",
pages = "176--180",
journal = "Cryobiology",
issn = "0011-2240",
publisher = "Academic Press Inc.",
number = "2",
}