Structure-specific recognition protein 1 facilitates microtubule growth and bundling required for mitosis

Shelya X. Zeng, Yanping Li, Yetao Jin, Qi Zhang, David M. Keller, Carolyn M. McQuaw, Eric Barklis, Stacie Stone, Maureen Hoatlin, Yingming Zhao, Hua Lu

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

Tight regulation of microtubule (MT) dynamics is essential for proper chromosome movement during mitosis. Here we show, using mammalian cells, that structure-specific recognition protein 1 (SSRP1) is a novel regulator of MT dynamics. SSRP1 colocalizes with the spindle and midbody MTs, and associates with MTs both in vitro and in vivo. Purified SSRP1 facilitates tubulin polymerization and MT bundling in vitro. Knockdown of SSRP1 inhibits the growth of MTs and leads to disorganized spindle structures, reduction of K-fibers and midbody fibers, disrupted chromosome movement, and attenuated cytokinesis in vivo. These results demonstrate that SSRP1 is crucial for MT growth and spindle assembly during mitosis.

Original languageEnglish (US)
Pages (from-to)935-947
Number of pages13
JournalMolecular and cellular biology
Volume30
Issue number4
DOIs
StatePublished - Feb 1 2010

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ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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