TY - JOUR
T1 - Structure of trichamide, a cyclic peptide from the bloom-forming cyanobacterium Tnchodesmium erythraeum, predicted from the genome sequence
AU - Sudek, Sebastian
AU - Haygood, Margo G.
AU - Youssef, Diaa T.A.
AU - Schmidt, Eric W.
PY - 2006/6
Y1 - 2006/6
N2 - A gene cluster for the biosynthesis of a new small cyclic peptide, dubbed trichamide, was discovered in the genome of the global, bloom-forming marine cyanobacterium Trichodesmium erythraeum ISM101 because of striking similarities to the previously characterized patellamide biosynthesis cluster. The tri cluster consists of a precursor peptide gene containing the amino acid sequence for mature trichamide, a putative heterocyclization gene, an oxidase, two proteases, and hypothetical genes. Based upon detailed sequence analysis, a structure was predicted for trichamide and confirmed by Fourier transform mass spectrometry. Trichamide consists of 11 amino acids, including two cysteine-derived thiazole groups, and is cyclized by an N-C terminal amide bond. As the first natural product reported from T. erythraeum, trichamide shows the power of genome mining in the prediction and discovery of new natural products.
AB - A gene cluster for the biosynthesis of a new small cyclic peptide, dubbed trichamide, was discovered in the genome of the global, bloom-forming marine cyanobacterium Trichodesmium erythraeum ISM101 because of striking similarities to the previously characterized patellamide biosynthesis cluster. The tri cluster consists of a precursor peptide gene containing the amino acid sequence for mature trichamide, a putative heterocyclization gene, an oxidase, two proteases, and hypothetical genes. Based upon detailed sequence analysis, a structure was predicted for trichamide and confirmed by Fourier transform mass spectrometry. Trichamide consists of 11 amino acids, including two cysteine-derived thiazole groups, and is cyclized by an N-C terminal amide bond. As the first natural product reported from T. erythraeum, trichamide shows the power of genome mining in the prediction and discovery of new natural products.
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U2 - 10.1128/AEM.00380-06
DO - 10.1128/AEM.00380-06
M3 - Article
C2 - 16751554
AN - SCOPUS:33745171769
SN - 0099-2240
VL - 72
SP - 4382
EP - 4387
JO - Applied and Environmental Microbiology
JF - Applied and Environmental Microbiology
IS - 6
ER -