Structure of trichamide, a cyclic peptide from the bloom-forming cyanobacterium Tnchodesmium erythraeum, predicted from the genome sequence

Sebastian Sudek, Margo G. Haygood, Diaa T.A. Youssef, Eric W. Schmidt

Research output: Contribution to journalArticle

103 Scopus citations

Abstract

A gene cluster for the biosynthesis of a new small cyclic peptide, dubbed trichamide, was discovered in the genome of the global, bloom-forming marine cyanobacterium Trichodesmium erythraeum ISM101 because of striking similarities to the previously characterized patellamide biosynthesis cluster. The tri cluster consists of a precursor peptide gene containing the amino acid sequence for mature trichamide, a putative heterocyclization gene, an oxidase, two proteases, and hypothetical genes. Based upon detailed sequence analysis, a structure was predicted for trichamide and confirmed by Fourier transform mass spectrometry. Trichamide consists of 11 amino acids, including two cysteine-derived thiazole groups, and is cyclized by an N-C terminal amide bond. As the first natural product reported from T. erythraeum, trichamide shows the power of genome mining in the prediction and discovery of new natural products.

Original languageEnglish (US)
Pages (from-to)4382-4387
Number of pages6
JournalApplied and Environmental Microbiology
Volume72
Issue number6
DOIs
StatePublished - Jun 2006

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

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