Structure of the human M2 muscarinic acetylcholine receptor gene and its promoter

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

The M2 muscarinic receptor inhibits the release of acetylcholine from cholinergic fibers in the lungs and elsewhere. In airway parasympathetic neurons, M2 receptor expression is decreased by viral infections and by interferon-γ, increasing actylcholine release. Dexamethasone increases M2 receptor expression, decreasing acetylcholine release. We carried out 5′ rapid amplification of cDNA ends beginning with mRNA from human heart and IMR32 human neuroblastoma cells. This demonstrated a 5′ UTR of 100 BP, corresponding to two sequences on chromosome 7, separated by a 22.6 kB intron. The splice acceptor site is at -45 relative to the initiating atg. The 3000 BP upstream of 5′ RACE product were subcloned into a pGL3 luciferase reporter vector. Deletional constructs were expressed in IMR32 cells. These demonstrated that 412 BP provided full expression of the reporter gene, and suggested a repressor element between -1848 and -1510.

Original languageEnglish (US)
Pages (from-to)87-92
Number of pages6
JournalGene
Volume271
Issue number1
DOIs
StatePublished - Jun 13 2001
Externally publishedYes

Keywords

  • Asthma
  • Glucocorticoid
  • Interferon
  • Neural
  • Parasympathetic

ASJC Scopus subject areas

  • Genetics

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