Structure of the gene encoding rat thyrotropin releasing hormone

S. L. Lee, K. Stewart, Richard Goodman

Research output: Contribution to journalArticle

113 Citations (Scopus)

Abstract

The gene encoding the hypothalamic peptide thyrotropin releasing hormone (TRH) was isolated from two rat genomic bacteriophage libraries. Southern blot analysis indicates that the gene is present as a single copy in the rat genome. The transcriptional unit is 2.6 kilobases in size and contains three exons interrupted by two introns of approximately 750 and 450 base pairs. Exon 1 encodes the 5'-untranslated region of the mRNA, exon 2 encodes the signal sequence and the majority of the amino-terminal peptide, and exon 3 encodes the remainder of the amino-terminal peptide, five copies of the TRH sequence, the carboxyl-terminal peptide, and the 3'-untranslated region of the mRNA. The general structure of the TRH gene resembles that of three other polypeptide hormone genes, preproopiomelanocortin, preproenkephalin A, and preproenkephalin B. Further sequence analysis revealed that characteristic promoter elements are present in the 5'-flanking region of the TRH gene. In addition, sequence homologies with the glucocorticoid receptor binding site, the GC box hexanucleotide, and a portion of the flanking region of the thyroid stimulating hormone β-subunit gene were also identified. These upstream sequences may be important for the regulation of TRH gene expression.

Original languageEnglish (US)
Pages (from-to)16604-16609
Number of pages6
JournalJournal of Biological Chemistry
Volume263
Issue number32
StatePublished - 1988
Externally publishedYes

Fingerprint

Gene encoding
Thyrotropin-Releasing Hormone
Rats
Genes
Exons
Peptides
Peptide Hormones
Pro-Opiomelanocortin
Messenger RNA
Bacteriophages
Genomic Library
5' Flanking Region
5' Untranslated Regions
Glucocorticoid Receptors
3' Untranslated Regions
Thyrotropin
Sequence Homology
Protein Sorting Signals
Southern Blotting
Gene expression

ASJC Scopus subject areas

  • Biochemistry

Cite this

Structure of the gene encoding rat thyrotropin releasing hormone. / Lee, S. L.; Stewart, K.; Goodman, Richard.

In: Journal of Biological Chemistry, Vol. 263, No. 32, 1988, p. 16604-16609.

Research output: Contribution to journalArticle

Lee, S. L. ; Stewart, K. ; Goodman, Richard. / Structure of the gene encoding rat thyrotropin releasing hormone. In: Journal of Biological Chemistry. 1988 ; Vol. 263, No. 32. pp. 16604-16609.
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