Structure of BT-3984, a member of the SusD/RagB family of nutrient-binding molecules

Constantina Bakolitsa; Qingping Xu; Christopher L. Rife; Polat Abdubek; Tamara Astakhova; Herbert L. Axelrod; Dennis Carlton; Connie Chen; Hsiu Ju Chiu; Thomas Clayton; Debanu Das; Marc C. Deller; Lian Duan; Kyle Ellrott; Carol L. Farr; Julie Feuerhelm; Joanna C. Grant; Anna Grzechnik; Gye Won Han; Lukasz Jaroszewski; Kevin K. Jin; Heath E. Klock; Mark W. Knuth; Piotr Kozbial; S. Sri Krishna; Abhinav Kumar; Winnie W. Lam; David Marciano; Daniel McMullan; Mitchell D. Miller; Andrew T. Morse; Edward Nigoghossian; Amanda Nopakun; Linda Okach; Christina Puckett; Ron Reyes; Henry J. Tien; Christine B. Trame; Henry Van Den Bedem; Dana Weekes; Keith O. Hodgson; John Wooley; Marc André Elsliger; Ashley M. Deacon; Adam Godzik; Scott A. Lesley; Ian A. Wilson

doi:10.1107/S1744309110032999

Structure of BT-3984, a member of the SusD/RagB family of nutrient-binding molecules

Constantina Bakolitsa, Qingping Xu, Christopher L. Rife, Polat Abdubek, Tamara Astakhova, Herbert L. Axelrod, Dennis Carlton, Connie Chen, Hsiu Ju Chiu, Thomas Clayton, Debanu Das, Marc C. Deller, Lian Duan, Kyle Ellrott, Carol L. Farr, Julie Feuerhelm, Joanna C. Grant, Anna Grzechnik, Gye Won Han, Lukasz JaroszewskiKevin K. Jin, Heath E. Klock, Mark W. Knuth, Piotr Kozbial, S. Sri Krishna, Abhinav Kumar, Winnie W. Lam, David Marciano, Daniel McMullan, Mitchell D. Miller, Andrew T. Morse, Edward Nigoghossian, Amanda Nopakun, Linda Okach, Christina Puckett, Ron Reyes, Henry J. Tien, Christine B. Trame, Henry Van Den Bedem, Dana Weekes, Keith O. Hodgson, John Wooley, Marc André Elsliger, Ashley M. Deacon, Adam Godzik, Scott A. Lesley, Ian A. Wilson

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

The crystal structure of the Bacteroides thetaiotaomicron protein BT-3984 was determined to a resolution of 1.7 Å and was the first structure to be determined from the extensive SusD family of polysaccharide-binding proteins. SusD is an essential component of the sus operon that defines the paradigm for glycan utilization in dominant members of the human gut microbiota. Structural analysis of BT-3984 revealed an N-terminal region containing several tetratricopeptide repeats (TPRs), while the signature C-terminal region is less structured and contains extensive loop regions. Sequence and structure analysis of BT-3984 suggests the presence of binding interfaces for other proteins from the polysaccharide-utilization complex.

Original language	English (US)
Pages (from-to)	1274-1280
Number of pages	7
Journal	Acta Crystallographica Section F: Structural Biology and Crystallization Communications
Volume	66
Issue number	10
DOIs	https://doi.org/10.1107/S1744309110032999
State	Published - Oct 2010
Externally published	Yes

Keywords

gut microbiome
metagenomics
starch-utilization system
structural genomics

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Genetics
Condensed Matter Physics

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10.1107/S1744309110032999

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Bakolitsa, C., Xu, Q., Rife, C. L., Abdubek, P., Astakhova, T., Axelrod, H. L., Carlton, D., Chen, C., Chiu, H. J., Clayton, T., Das, D., Deller, M. C., Duan, L., Ellrott, K., Farr, C. L., Feuerhelm, J., Grant, J. C., Grzechnik, A., Han, G. W., ... Wilson, I. A. (2010). Structure of BT-3984, a member of the SusD/RagB family of nutrient-binding molecules. Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 66(10), 1274-1280. https://doi.org/10.1107/S1744309110032999

Bakolitsa, C, Xu, Q, Rife, CL, Abdubek, P, Astakhova, T, Axelrod, HL, Carlton, D, Chen, C, Chiu, HJ, Clayton, T, Das, D, Deller, MC, Duan, L, Ellrott, K, Farr, CL, Feuerhelm, J, Grant, JC, Grzechnik, A, Han, GW, Jaroszewski, L, Jin, KK, Klock, HE, Knuth, MW, Kozbial, P, Krishna, SS, Kumar, A, Lam, WW, Marciano, D, McMullan, D, Miller, MD, Morse, AT, Nigoghossian, E, Nopakun, A, Okach, L, Puckett, C, Reyes, R, Tien, HJ, Trame, CB, Van Den Bedem, H, Weekes, D, Hodgson, KO, Wooley, J, Elsliger, MA, Deacon, AM, Godzik, A, Lesley, SA & Wilson, IA 2010, 'Structure of BT-3984, a member of the SusD/RagB family of nutrient-binding molecules', Acta Crystallographica Section F: Structural Biology and Crystallization Communications, vol. 66, no. 10, pp. 1274-1280. https://doi.org/10.1107/S1744309110032999

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title = "Structure of BT-3984, a member of the SusD/RagB family of nutrient-binding molecules",

abstract = "The crystal structure of the Bacteroides thetaiotaomicron protein BT-3984 was determined to a resolution of 1.7 {\AA} and was the first structure to be determined from the extensive SusD family of polysaccharide-binding proteins. SusD is an essential component of the sus operon that defines the paradigm for glycan utilization in dominant members of the human gut microbiota. Structural analysis of BT-3984 revealed an N-terminal region containing several tetratricopeptide repeats (TPRs), while the signature C-terminal region is less structured and contains extensive loop regions. Sequence and structure analysis of BT-3984 suggests the presence of binding interfaces for other proteins from the polysaccharide-utilization complex.",

keywords = "gut microbiome, metagenomics, starch-utilization system, structural genomics",

author = "Constantina Bakolitsa and Qingping Xu and Rife, {Christopher L.} and Polat Abdubek and Tamara Astakhova and Axelrod, {Herbert L.} and Dennis Carlton and Connie Chen and Chiu, {Hsiu Ju} and Thomas Clayton and Debanu Das and Deller, {Marc C.} and Lian Duan and Kyle Ellrott and Farr, {Carol L.} and Julie Feuerhelm and Grant, {Joanna C.} and Anna Grzechnik and Han, {Gye Won} and Lukasz Jaroszewski and Jin, {Kevin K.} and Klock, {Heath E.} and Knuth, {Mark W.} and Piotr Kozbial and Krishna, {S. Sri} and Abhinav Kumar and Lam, {Winnie W.} and David Marciano and Daniel McMullan and Miller, {Mitchell D.} and Morse, {Andrew T.} and Edward Nigoghossian and Amanda Nopakun and Linda Okach and Christina Puckett and Ron Reyes and Tien, {Henry J.} and Trame, {Christine B.} and {Van Den Bedem}, Henry and Dana Weekes and Hodgson, {Keith O.} and John Wooley and Elsliger, {Marc Andr{\'e}} and Deacon, {Ashley M.} and Adam Godzik and Lesley, {Scott A.} and Wilson, {Ian A.}",

year = "2010",

month = oct,

doi = "10.1107/S1744309110032999",

language = "English (US)",

volume = "66",

pages = "1274--1280",

journal = "Acta Crystallographica Section F: Structural Biology and Crystallization Communications",

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T1 - Structure of BT-3984, a member of the SusD/RagB family of nutrient-binding molecules

AU - Bakolitsa, Constantina

AU - Xu, Qingping

AU - Rife, Christopher L.

AU - Abdubek, Polat

AU - Astakhova, Tamara

AU - Axelrod, Herbert L.

AU - Carlton, Dennis

AU - Chen, Connie

AU - Chiu, Hsiu Ju

AU - Clayton, Thomas

AU - Das, Debanu

AU - Deller, Marc C.

AU - Duan, Lian

AU - Ellrott, Kyle

AU - Farr, Carol L.

AU - Feuerhelm, Julie

AU - Grant, Joanna C.

AU - Grzechnik, Anna

AU - Han, Gye Won

AU - Jaroszewski, Lukasz

AU - Jin, Kevin K.

AU - Klock, Heath E.

AU - Knuth, Mark W.

AU - Kozbial, Piotr

AU - Krishna, S. Sri

AU - Kumar, Abhinav

AU - Lam, Winnie W.

AU - Marciano, David

AU - McMullan, Daniel

AU - Miller, Mitchell D.

AU - Morse, Andrew T.

AU - Nigoghossian, Edward

AU - Nopakun, Amanda

AU - Okach, Linda

AU - Puckett, Christina

AU - Reyes, Ron

AU - Tien, Henry J.

AU - Trame, Christine B.

AU - Van Den Bedem, Henry

AU - Weekes, Dana

AU - Hodgson, Keith O.

AU - Wooley, John

AU - Elsliger, Marc André

AU - Deacon, Ashley M.

AU - Godzik, Adam

AU - Lesley, Scott A.

AU - Wilson, Ian A.

PY - 2010/10

Y1 - 2010/10

N2 - The crystal structure of the Bacteroides thetaiotaomicron protein BT-3984 was determined to a resolution of 1.7 Å and was the first structure to be determined from the extensive SusD family of polysaccharide-binding proteins. SusD is an essential component of the sus operon that defines the paradigm for glycan utilization in dominant members of the human gut microbiota. Structural analysis of BT-3984 revealed an N-terminal region containing several tetratricopeptide repeats (TPRs), while the signature C-terminal region is less structured and contains extensive loop regions. Sequence and structure analysis of BT-3984 suggests the presence of binding interfaces for other proteins from the polysaccharide-utilization complex.

AB - The crystal structure of the Bacteroides thetaiotaomicron protein BT-3984 was determined to a resolution of 1.7 Å and was the first structure to be determined from the extensive SusD family of polysaccharide-binding proteins. SusD is an essential component of the sus operon that defines the paradigm for glycan utilization in dominant members of the human gut microbiota. Structural analysis of BT-3984 revealed an N-terminal region containing several tetratricopeptide repeats (TPRs), while the signature C-terminal region is less structured and contains extensive loop regions. Sequence and structure analysis of BT-3984 suggests the presence of binding interfaces for other proteins from the polysaccharide-utilization complex.

KW - gut microbiome

KW - metagenomics

KW - starch-utilization system

KW - structural genomics

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U2 - 10.1107/S1744309110032999

DO - 10.1107/S1744309110032999

M3 - Article

C2 - 20944222

AN - SCOPUS:77958033449

SN - 1744-3091

VL - 66

SP - 1274

EP - 1280

JO - Acta Crystallographica Section F: Structural Biology and Crystallization Communications

JF - Acta Crystallographica Section F: Structural Biology and Crystallization Communications

IS - 10

ER -

Structure of BT-3984, a member of the SusD/RagB family of nutrient-binding molecules

Abstract

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