Structure of BT-3984, a member of the SusD/RagB family of nutrient-binding molecules

Constantina Bakolitsa, Qingping Xu, Christopher L. Rife, Polat Abdubek, Tamara Astakhova, Herbert L. Axelrod, Dennis Carlton, Connie Chen, Hsiu Ju Chiu, Thomas Clayton, Debanu Das, Marc C. Deller, Lian Duan, Kyle Ellrott, Carol L. Farr, Julie Feuerhelm, Joanna C. Grant, Anna Grzechnik, Gye Won Han, Lukasz Jaroszewski & 27 others Kevin K. Jin, Heath E. Klock, Mark W. Knuth, Piotr Kozbial, S. Sri Krishna, Abhinav Kumar, Winnie W. Lam, David Marciano, Daniel McMullan, Mitchell D. Miller, Andrew T. Morse, Edward Nigoghossian, Amanda Nopakun, Linda Okach, Christina Puckett, Ron Reyes, Henry J. Tien, Christine B. Trame, Henry Van Den Bedem, Dana Weekes, Keith O. Hodgson, John Wooley, Marc André Elsliger, Ashley M. Deacon, Adam Godzik, Scott A. Lesley, Ian A. Wilson

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The crystal structure of the Bacteroides thetaiotaomicron protein BT-3984 was determined to a resolution of 1.7 Å and was the first structure to be determined from the extensive SusD family of polysaccharide-binding proteins. SusD is an essential component of the sus operon that defines the paradigm for glycan utilization in dominant members of the human gut microbiota. Structural analysis of BT-3984 revealed an N-terminal region containing several tetratricopeptide repeats (TPRs), while the signature C-terminal region is less structured and contains extensive loop regions. Sequence and structure analysis of BT-3984 suggests the presence of binding interfaces for other proteins from the polysaccharide-utilization complex.

Original languageEnglish (US)
Pages (from-to)1274-1280
Number of pages7
JournalActa Crystallographica Section F: Structural Biology and Crystallization Communications
Volume66
Issue number10
DOIs
StatePublished - Oct 2010
Externally publishedYes

Fingerprint

nutrients
Nutrients
Polysaccharides
polysaccharides
proteins
Food
Molecules
molecules
Operon
structural analysis
Structural analysis
Sequence Analysis
Carrier Proteins
Proteins
Crystal structure
signatures
crystal structure

Keywords

  • gut microbiome
  • metagenomics
  • starch-utilization system
  • structural genomics

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Structural Biology
  • Genetics
  • Condensed Matter Physics

Cite this

Structure of BT-3984, a member of the SusD/RagB family of nutrient-binding molecules. / Bakolitsa, Constantina; Xu, Qingping; Rife, Christopher L.; Abdubek, Polat; Astakhova, Tamara; Axelrod, Herbert L.; Carlton, Dennis; Chen, Connie; Chiu, Hsiu Ju; Clayton, Thomas; Das, Debanu; Deller, Marc C.; Duan, Lian; Ellrott, Kyle; Farr, Carol L.; Feuerhelm, Julie; Grant, Joanna C.; Grzechnik, Anna; Han, Gye Won; Jaroszewski, Lukasz; Jin, Kevin K.; Klock, Heath E.; Knuth, Mark W.; Kozbial, Piotr; Krishna, S. Sri; Kumar, Abhinav; Lam, Winnie W.; Marciano, David; McMullan, Daniel; Miller, Mitchell D.; Morse, Andrew T.; Nigoghossian, Edward; Nopakun, Amanda; Okach, Linda; Puckett, Christina; Reyes, Ron; Tien, Henry J.; Trame, Christine B.; Van Den Bedem, Henry; Weekes, Dana; Hodgson, Keith O.; Wooley, John; Elsliger, Marc André; Deacon, Ashley M.; Godzik, Adam; Lesley, Scott A.; Wilson, Ian A.

In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications, Vol. 66, No. 10, 10.2010, p. 1274-1280.

Research output: Contribution to journalArticle

Bakolitsa, C, Xu, Q, Rife, CL, Abdubek, P, Astakhova, T, Axelrod, HL, Carlton, D, Chen, C, Chiu, HJ, Clayton, T, Das, D, Deller, MC, Duan, L, Ellrott, K, Farr, CL, Feuerhelm, J, Grant, JC, Grzechnik, A, Han, GW, Jaroszewski, L, Jin, KK, Klock, HE, Knuth, MW, Kozbial, P, Krishna, SS, Kumar, A, Lam, WW, Marciano, D, McMullan, D, Miller, MD, Morse, AT, Nigoghossian, E, Nopakun, A, Okach, L, Puckett, C, Reyes, R, Tien, HJ, Trame, CB, Van Den Bedem, H, Weekes, D, Hodgson, KO, Wooley, J, Elsliger, MA, Deacon, AM, Godzik, A, Lesley, SA & Wilson, IA 2010, 'Structure of BT-3984, a member of the SusD/RagB family of nutrient-binding molecules', Acta Crystallographica Section F: Structural Biology and Crystallization Communications, vol. 66, no. 10, pp. 1274-1280. https://doi.org/10.1107/S1744309110032999
Bakolitsa, Constantina ; Xu, Qingping ; Rife, Christopher L. ; Abdubek, Polat ; Astakhova, Tamara ; Axelrod, Herbert L. ; Carlton, Dennis ; Chen, Connie ; Chiu, Hsiu Ju ; Clayton, Thomas ; Das, Debanu ; Deller, Marc C. ; Duan, Lian ; Ellrott, Kyle ; Farr, Carol L. ; Feuerhelm, Julie ; Grant, Joanna C. ; Grzechnik, Anna ; Han, Gye Won ; Jaroszewski, Lukasz ; Jin, Kevin K. ; Klock, Heath E. ; Knuth, Mark W. ; Kozbial, Piotr ; Krishna, S. Sri ; Kumar, Abhinav ; Lam, Winnie W. ; Marciano, David ; McMullan, Daniel ; Miller, Mitchell D. ; Morse, Andrew T. ; Nigoghossian, Edward ; Nopakun, Amanda ; Okach, Linda ; Puckett, Christina ; Reyes, Ron ; Tien, Henry J. ; Trame, Christine B. ; Van Den Bedem, Henry ; Weekes, Dana ; Hodgson, Keith O. ; Wooley, John ; Elsliger, Marc André ; Deacon, Ashley M. ; Godzik, Adam ; Lesley, Scott A. ; Wilson, Ian A. / Structure of BT-3984, a member of the SusD/RagB family of nutrient-binding molecules. In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications. 2010 ; Vol. 66, No. 10. pp. 1274-1280.
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AU - Bakolitsa, Constantina

AU - Xu, Qingping

AU - Rife, Christopher L.

AU - Abdubek, Polat

AU - Astakhova, Tamara

AU - Axelrod, Herbert L.

AU - Carlton, Dennis

AU - Chen, Connie

AU - Chiu, Hsiu Ju

AU - Clayton, Thomas

AU - Das, Debanu

AU - Deller, Marc C.

AU - Duan, Lian

AU - Ellrott, Kyle

AU - Farr, Carol L.

AU - Feuerhelm, Julie

AU - Grant, Joanna C.

AU - Grzechnik, Anna

AU - Han, Gye Won

AU - Jaroszewski, Lukasz

AU - Jin, Kevin K.

AU - Klock, Heath E.

AU - Knuth, Mark W.

AU - Kozbial, Piotr

AU - Krishna, S. Sri

AU - Kumar, Abhinav

AU - Lam, Winnie W.

AU - Marciano, David

AU - McMullan, Daniel

AU - Miller, Mitchell D.

AU - Morse, Andrew T.

AU - Nigoghossian, Edward

AU - Nopakun, Amanda

AU - Okach, Linda

AU - Puckett, Christina

AU - Reyes, Ron

AU - Tien, Henry J.

AU - Trame, Christine B.

AU - Van Den Bedem, Henry

AU - Weekes, Dana

AU - Hodgson, Keith O.

AU - Wooley, John

AU - Elsliger, Marc André

AU - Deacon, Ashley M.

AU - Godzik, Adam

AU - Lesley, Scott A.

AU - Wilson, Ian A.

PY - 2010/10

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N2 - The crystal structure of the Bacteroides thetaiotaomicron protein BT-3984 was determined to a resolution of 1.7 Å and was the first structure to be determined from the extensive SusD family of polysaccharide-binding proteins. SusD is an essential component of the sus operon that defines the paradigm for glycan utilization in dominant members of the human gut microbiota. Structural analysis of BT-3984 revealed an N-terminal region containing several tetratricopeptide repeats (TPRs), while the signature C-terminal region is less structured and contains extensive loop regions. Sequence and structure analysis of BT-3984 suggests the presence of binding interfaces for other proteins from the polysaccharide-utilization complex.

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KW - gut microbiome

KW - metagenomics

KW - starch-utilization system

KW - structural genomics

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