Structure of a precursor to human pancreatic polypeptide

A. B. Leiter, H. T. Keutmann, Richard Goodman

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

We have isolated mRNA from a human pancreatic islet cell tumor and have identified among the cell-free translation products a precursor of pancreatic polypeptide with an approximate M(r) = 11,000. Recombinant DNA molecules encoding this precursor were selected from a cDNA library prepared from the islet tumor mRNA. From the nucleotide sequences of cDNAs encoding the precursor, we have deduced the complete amino acid sequence of pre-propancreatic polypeptide. These sequences encode a protein consisting of 95 amino acid residues with a M(r) = 10,432. The sequence of human pancreatic polypeptide occurs in the middle of the precursors and is flanked at its carboxyl terminus by a 27-amino acid sequence which is similar to a peptide previously isolated from canine pancreatic islets. At the amino terminus of the precursor is a probable leader sequence which is rich in hydrophobic residues. A smaller pancreatic polypeptide-related protein was generated in cell-free translations of mRNA supplemented with microsomal membranes. Sequential Edman degradations of this smaller peptide indicate that the sequence of pancreatic polypeptide is located at the amino terminus of the prohormone.

Original languageEnglish (US)
Pages (from-to)14702-14705
Number of pages4
JournalJournal of Biological Chemistry
Volume259
Issue number23
StatePublished - 1984
Externally publishedYes

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Pancreatic Polypeptide
Amino Acids
Messenger RNA
Peptides
Tumors
Amino Acid Sequence
Recombinant DNA
Protein Biosynthesis
Islets of Langerhans
Gene Library
Canidae
Proteins
Nucleotides
Complementary DNA
Membranes
Degradation
Molecules
human PPY protein
Neoplasms

ASJC Scopus subject areas

  • Biochemistry

Cite this

Structure of a precursor to human pancreatic polypeptide. / Leiter, A. B.; Keutmann, H. T.; Goodman, Richard.

In: Journal of Biological Chemistry, Vol. 259, No. 23, 1984, p. 14702-14705.

Research output: Contribution to journalArticle

Leiter, AB, Keutmann, HT & Goodman, R 1984, 'Structure of a precursor to human pancreatic polypeptide', Journal of Biological Chemistry, vol. 259, no. 23, pp. 14702-14705.
Leiter, A. B. ; Keutmann, H. T. ; Goodman, Richard. / Structure of a precursor to human pancreatic polypeptide. In: Journal of Biological Chemistry. 1984 ; Vol. 259, No. 23. pp. 14702-14705.
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