Structure of a four-way bridged ParB-DNA complex provides insight into P1 segrosome assembly

Maria A. Schumacher, Andre Mansoor, Barbara E. Funnell

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The plasmid partition process is essential for plasmid propagation and is mediated by par systems, consisting of centromere-like sites and two proteins, ParA and ParB. In the first step of partition by the archetypical P1 system, ParB binds a complicated centromere-like site to form a large nucleoprotein segrosome. ParB is a dimeric DNA-binding protein that can bridge between both A-boxes and B-boxes located on the centromere. Its helix-turn-helix domains bind A-boxes and the dimer domain binds B-boxes. Binding of the first ParB dimer nucleates the remaining ParB molecules onto the centromere site, which somehow leads to the formation of a condensed segrosome superstructure. To further understand this unique DNA spreading capability of ParB, we crystallized and determined the structure of a 1:2 ParB-(142-333):A3-B2-box complex to 3.35 Å resolution. The structure reveals a remarkable four-way, protein-DNA bridged complex in which both ParB helix-turn-helix domains simultaneously bind adjacent A-boxes and the dimer domain bridges between two B-boxes. The multibridging capability and the novel dimer domain-B-box interaction, which juxtaposes the DNA sites close in space, suggests a mechanism for the formation of the wrapped solenoid-like segrosome superstructure. This multibridging capability of ParB is likely critical in its partition complex formation and pairing functions.

Original languageEnglish (US)
Pages (from-to)10456-10464
Number of pages9
JournalJournal of Biological Chemistry
Volume282
Issue number14
DOIs
StatePublished - Apr 6 2007

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Centromere
Dimers
DNA
Plasmids
Nucleoproteins
Solenoids
DNA-Binding Proteins
Proteins
Molecules

ASJC Scopus subject areas

  • Biochemistry

Cite this

Structure of a four-way bridged ParB-DNA complex provides insight into P1 segrosome assembly. / Schumacher, Maria A.; Mansoor, Andre; Funnell, Barbara E.

In: Journal of Biological Chemistry, Vol. 282, No. 14, 06.04.2007, p. 10456-10464.

Research output: Contribution to journalArticle

Schumacher, Maria A. ; Mansoor, Andre ; Funnell, Barbara E. / Structure of a four-way bridged ParB-DNA complex provides insight into P1 segrosome assembly. In: Journal of Biological Chemistry. 2007 ; Vol. 282, No. 14. pp. 10456-10464.
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