Structure and organization of the chicken H2B histone gene family

David Grandy, Jerry B. Dodgson

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The results of Southern blotting experiments confirm that the chicken H2B histone gene family contains eight highly homologous members. One or two more sequences which are considerably divergent from the others appear to exist in the chicken genome. Seven of the eight H2B genes have been cloned and sequenced. All seven genes fall in two histone gene clusters, but no common arrangement exists for the clusters themselves. Three different H2B protein variants are encoded by these seven genes. The nucleotide sequence homology among the genes within their coding sequences appears to exceed that required for the corresponding protein sequences, suggesting that histone H2B mRNA sequence and structure are both selected during evolution. An analysis of the 5′ flanking sequence data reveals that these genes possess CCAAT and TATA boxes, elements commonly associated with genes transcribed by RNA polymerase II. In addition, these genes all share an H2B-specific element of the form: ATTTGCATA. The 3′ sequences of these genes contain the hyphenated symmetrical dyad homology and downstream purine-rich sequence shared by histone genes in general.

Original languageEnglish (US)
Pages (from-to)1063-1080
Number of pages18
JournalNucleic Acids Research
Volume15
Issue number3
DOIs
StatePublished - Feb 11 1987
Externally publishedYes

Fingerprint

Histones
Chickens
Genes
Gene
Homology
Family
Chicken
TATA Box
RNA Polymerase II
5' Flanking Region
Proteins
Multigene Family
Sequence Homology
Southern Blotting
Protein Sequence
Nucleotides
Messenger RNA
RNA
Arrangement
Exceed

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)
  • Genetics

Cite this

Structure and organization of the chicken H2B histone gene family. / Grandy, David; Dodgson, Jerry B.

In: Nucleic Acids Research, Vol. 15, No. 3, 11.02.1987, p. 1063-1080.

Research output: Contribution to journalArticle

Grandy, David ; Dodgson, Jerry B. / Structure and organization of the chicken H2B histone gene family. In: Nucleic Acids Research. 1987 ; Vol. 15, No. 3. pp. 1063-1080.
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