Structure and function of kidney water channels

A. S. Verkman, L. B. Shi, A. Frigeri, H. Hasegawa, J. Farinas, A. Mitra, William Skach, D. Brown, A. N. Van Hoek, T. Ma

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

There is now firm evidence that water transporting proteins are expressed in renal and extrarenal tissues. In the kidney, proximal-type (CHIP28) and collecting duct (WCH-CD) water channels have been identified. We have cloned three kidney cDNAs with homology to the water channel (aquaporin) family, including a mercurial-insensitive water channel (MIWC), and a glycerol-transporting protein (GLIP) in collecting duct basolateral membrane. To elucidate water transporting mechanisms, a series of molecular and spectroscopic studies were carried out on purified CHIP28 protein and expressed chimeric and mutated CHIP28 cDNAs. The results indicate that CHIP28 transports water selectively, that CHIP28 monomers are assembled in membranes as tetramers, but that individual monomers function independently. Monomers contain multiple membrane-spanning helical domains. Based on these data and recent electron crystallography results, a model for water transport is proposed in which water moves through narrow pores located within individual CHIP28 monomers.

Original languageEnglish (US)
Pages (from-to)1069-1081
Number of pages13
JournalKidney International
Volume48
Issue number4
StatePublished - 1995
Externally publishedYes

Fingerprint

Aquaporins
Kidney
Water
Membranes
Complementary DNA
Aquaporin 1
Crystallography
Glycerol
Proteins
Electrons

ASJC Scopus subject areas

  • Nephrology

Cite this

Verkman, A. S., Shi, L. B., Frigeri, A., Hasegawa, H., Farinas, J., Mitra, A., ... Ma, T. (1995). Structure and function of kidney water channels. Kidney International, 48(4), 1069-1081.

Structure and function of kidney water channels. / Verkman, A. S.; Shi, L. B.; Frigeri, A.; Hasegawa, H.; Farinas, J.; Mitra, A.; Skach, William; Brown, D.; Van Hoek, A. N.; Ma, T.

In: Kidney International, Vol. 48, No. 4, 1995, p. 1069-1081.

Research output: Contribution to journalArticle

Verkman, AS, Shi, LB, Frigeri, A, Hasegawa, H, Farinas, J, Mitra, A, Skach, W, Brown, D, Van Hoek, AN & Ma, T 1995, 'Structure and function of kidney water channels', Kidney International, vol. 48, no. 4, pp. 1069-1081.
Verkman AS, Shi LB, Frigeri A, Hasegawa H, Farinas J, Mitra A et al. Structure and function of kidney water channels. Kidney International. 1995;48(4):1069-1081.
Verkman, A. S. ; Shi, L. B. ; Frigeri, A. ; Hasegawa, H. ; Farinas, J. ; Mitra, A. ; Skach, William ; Brown, D. ; Van Hoek, A. N. ; Ma, T. / Structure and function of kidney water channels. In: Kidney International. 1995 ; Vol. 48, No. 4. pp. 1069-1081.
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AU - Mitra, A.

AU - Skach, William

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AU - Van Hoek, A. N.

AU - Ma, T.

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