Structure activity relationships of an exotoxin of Pseudomonas aeruginosa

M. L. Vasil, David Kabat, B. H. Iglewski

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

The relation of the structure of P. aeruginosa exotoxin A (PA toxin) to its enzymatic activity (adenosine 5'-diphosphate-ribosyl transferase) in vitro and to its toxicity in vivo was examined. PA toxin is produced as a single polypeptide chain with a molecular weight of about 71,500. PA toxin is produced by Pseudomonas as a toxic proenzyme that lacks enzymatic activity. Adenosine 5'-diphosphate- ribosyl transferase activity is expressed when the molecule is denatured and reduced or when it is cleaved by Pseudomonas proteases to yield an enzymatically active 27,000-dalton fragment (fragment a). A 45,000-dalton protein is tentatively identified as the enzymatically inactive fragment b of PA toxin. Enzymatically active forms of the toxin lack toxicity for mouse L-cells or mouse lethality. Thus, it is concluded that the native toxin proenzyme is required for toxicity and that a structural rearrangement must precede its intracellular activity.

Original languageEnglish (US)
Pages (from-to)353-361
Number of pages9
JournalInfection and Immunity
Volume16
Issue number1
StatePublished - 1977

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Exotoxins
Enzyme Precursors
Structure-Activity Relationship
Transferases
Pseudomonas
Adenosine Diphosphate
Pseudomonas aeruginosa
Poisons
Peptide Hydrolases
Molecular Weight
Peptides
Proteins
Pseudomonas aeruginosa toxA protein
In Vitro Techniques

ASJC Scopus subject areas

  • Immunology

Cite this

Vasil, M. L., Kabat, D., & Iglewski, B. H. (1977). Structure activity relationships of an exotoxin of Pseudomonas aeruginosa. Infection and Immunity, 16(1), 353-361.

Structure activity relationships of an exotoxin of Pseudomonas aeruginosa. / Vasil, M. L.; Kabat, David; Iglewski, B. H.

In: Infection and Immunity, Vol. 16, No. 1, 1977, p. 353-361.

Research output: Contribution to journalArticle

Vasil, ML, Kabat, D & Iglewski, BH 1977, 'Structure activity relationships of an exotoxin of Pseudomonas aeruginosa', Infection and Immunity, vol. 16, no. 1, pp. 353-361.
Vasil, M. L. ; Kabat, David ; Iglewski, B. H. / Structure activity relationships of an exotoxin of Pseudomonas aeruginosa. In: Infection and Immunity. 1977 ; Vol. 16, No. 1. pp. 353-361.
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