Structural similarities between MutT and the C-terminal domain of MutY

David E. Volk, Paul G. House, Varatharasa Thiviyanathan, Bruce A. Luxon, Shanmin Zhang, Robert (Stephen) Lloyd, David G. Gorenstein

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

One of the functions of MutY from Escherchia coli is removal of adenine mispaired with 7,8-dihydro-8-oxoguanine (8-oxoG), a common lesion in oxidatively damaged DNA. MutY is composed of two domains: the larger N- terminal domain (p26) contains the catalytic properties of the enzyme while the C-terminal domain (p13) affects substrate recognition and enzyme turnover. On the basis of sequence analyses, it has been recently suggested that the C-terminal domain is distantly related to MutT, a dNTPase which hydrolyzes 8-oxo-dGTP [Noll et al. (1999) Biochemistry 38, 6374-6279]. We have studied the solution structure of the C-terminal domain of MutY by NMR and find striking similarity with the reported solution structure of MutT. Despite low sequence identity between the two proteins, they have similar secondary structure and topology. The C-terminal domain of MutY is composed of two α-helices and five β-strands. The NOESY data indicate that the protein has two β-sheets. MutT is also a mixed α/β protein with two helices and two β-sheets composed of five strands. The secondary structure elements are similarly arranged in the two proteins.

Original languageEnglish (US)
Pages (from-to)7331-7336
Number of pages6
JournalBiochemistry
Volume39
Issue number25
DOIs
StatePublished - Jun 27 2000
Externally publishedYes

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Proteins
Biochemistry
Adenine
Enzymes
Sequence Analysis
Nuclear magnetic resonance
Topology
DNA
Substrates
8-oxodeoxyguanosine triphosphate
7,8-dihydro-8-oxoguanine

ASJC Scopus subject areas

  • Biochemistry

Cite this

Volk, D. E., House, P. G., Thiviyanathan, V., Luxon, B. A., Zhang, S., Lloyd, R. S., & Gorenstein, D. G. (2000). Structural similarities between MutT and the C-terminal domain of MutY. Biochemistry, 39(25), 7331-7336. https://doi.org/10.1021/bi000416p

Structural similarities between MutT and the C-terminal domain of MutY. / Volk, David E.; House, Paul G.; Thiviyanathan, Varatharasa; Luxon, Bruce A.; Zhang, Shanmin; Lloyd, Robert (Stephen); Gorenstein, David G.

In: Biochemistry, Vol. 39, No. 25, 27.06.2000, p. 7331-7336.

Research output: Contribution to journalArticle

Volk, DE, House, PG, Thiviyanathan, V, Luxon, BA, Zhang, S, Lloyd, RS & Gorenstein, DG 2000, 'Structural similarities between MutT and the C-terminal domain of MutY', Biochemistry, vol. 39, no. 25, pp. 7331-7336. https://doi.org/10.1021/bi000416p
Volk DE, House PG, Thiviyanathan V, Luxon BA, Zhang S, Lloyd RS et al. Structural similarities between MutT and the C-terminal domain of MutY. Biochemistry. 2000 Jun 27;39(25):7331-7336. https://doi.org/10.1021/bi000416p
Volk, David E. ; House, Paul G. ; Thiviyanathan, Varatharasa ; Luxon, Bruce A. ; Zhang, Shanmin ; Lloyd, Robert (Stephen) ; Gorenstein, David G. / Structural similarities between MutT and the C-terminal domain of MutY. In: Biochemistry. 2000 ; Vol. 39, No. 25. pp. 7331-7336.
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