Structural characterization and immunohistochemical localization of receptors for insulin-like growth factor II in the rat pituitary gland

Ian Ocrant, Karen L. Valentino, Andrew R. Hoffman, Raymond L. Hintz, Darrell M. Wilson, Ronald (Ron) Rosenfeld

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Insulin-like growth factor N (IGF-II) receptors were detected, localized, and structurally characterized in rat pituitary tissue sections and cultures of dispersed pituitary cells by immurtohistochemtstry, and affinity labeling with pel electrophoresis. Using highly specific antisera against IGF-II receptors (type 2 IGF receptors) and somatotropin (GH), intense type 2 receptor immunoreactivity was detected in both anterior and intermediate pituitary lobes. In anterior pituitary sections and cultures, type 2 receptor immunoreactivity colocalized to most GH-immunoreactive cells (somatc-tropes), as well as cells which did not contain GH. Intermediate lobe immunoreactivity was uniformly distributed throughout the parenchyma. In both anterior and intermediate pituitary lobes, type 2 receptor immunoreactivity was predominately localized to the plasma membrane of labeled cells, with little or no cytoplasmic labeling. GH immunoreactivity, on the other hand, was intracellular. Affinity labeling of microsomal membranes from anterior and neurointermediate pituitary tissues with '-M-IGF-II disclosed classical 230k type 2 receptors. The magnitude ofaffinity cross-linking from both lobes was similar to that of rat liver, indicating pituitary tissues, like liver tissue, are rich sources of type 2 receptors. These results suggest possible roles for IGF-II and the type 2 receptor in the regulation of synthesis or secretion of pituitary trophic hormones, including GH and proopiomelanocortin gene products.

Original languageEnglish (US)
Pages (from-to)248-254
Number of pages7
JournalNeuroendocrinology
Volume49
Issue number3
DOIs
StatePublished - 1989
Externally publishedYes

Fingerprint

IGF Type 2 Receptor
Pituitary Gland
Intermediate Pituitary Gland
Anterior Pituitary Gland
Pro-Opiomelanocortin
Insulin-Like Growth Factor II
Pituitary Hormones
Liver
Somatomedins
Growth Hormone
Electrophoresis
Immune Sera
Cell Membrane
rat insulin-like growth factor II
Membranes
Genes

Keywords

  • Growth factor receptors
  • Growth factors
  • Insulin-like growth factor M
  • Insulin-like growth factor receptors
  • Insulin-like growth factors
  • Pituitary regulation

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism
  • Cellular and Molecular Neuroscience
  • Endocrine and Autonomic Systems
  • Neuroscience(all)

Cite this

Structural characterization and immunohistochemical localization of receptors for insulin-like growth factor II in the rat pituitary gland. / Ocrant, Ian; Valentino, Karen L.; Hoffman, Andrew R.; Hintz, Raymond L.; Wilson, Darrell M.; Rosenfeld, Ronald (Ron).

In: Neuroendocrinology, Vol. 49, No. 3, 1989, p. 248-254.

Research output: Contribution to journalArticle

Ocrant, Ian ; Valentino, Karen L. ; Hoffman, Andrew R. ; Hintz, Raymond L. ; Wilson, Darrell M. ; Rosenfeld, Ronald (Ron). / Structural characterization and immunohistochemical localization of receptors for insulin-like growth factor II in the rat pituitary gland. In: Neuroendocrinology. 1989 ; Vol. 49, No. 3. pp. 248-254.
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