TY - JOUR
T1 - Structural changes in thestrial blood–labyrinth barrier of aged C57BL/6 mice
AU - Neng, Lingling
AU - Zhang, Jinhui
AU - Yang, Ju
AU - Zhang, Fei
AU - Lopez, Ivan A.
AU - Dong, Mingmin
AU - Shi, Xiaorui
N1 - Funding Information:
This work was supported by the National Institutes of Health (grants NIH NIDCD R01-DC010844 to X.S., DC R21DC1239801 to X.S. and NIHP30-DC005983), the National Key Basic Research Program of China (no. 2014CB943001) and the National Natural Science Foundation of China (no. 81120108009).
Publisher Copyright:
© 2015, Springer-Verlag Berlin Heidelberg.
PY - 2015/9/28
Y1 - 2015/9/28
N2 - Tight control over cochlear blood flow (CoBF) and the blood-labyrinth barrier (BLB) in the striavascularis is critical for maintaining the ionic, fluid and energy balance necessary for hearing function. Inefficient CoBF and disruption of BLB integrity have long been considered major etiologic factors in a variety of hearing disorders. In this study, we investigate structural changes in the BLB of the striavascularis in age-graded C57BL/6 mice (1 to 21 months) with a focus on changes in two blood barrier accessory cells, namely pericytes (PCs) and perivascular-resident macrophage-like melanocytes (PVM/Ms). Decreased capillary density was detectable at 6 months, with significant capillary degeneration seen in 9- to 21-month-old mice. Reduced capillary density was highly correlated with lower numbers of PCs and PVM/Ms. “Drop-out” of PCs and “activation” of PVM/Ms were seen at 6 months, with drastic changes being observed by 21 months. With newly established in vitro three-dimensional cell-based co-culture models, we demonstrate that PCs and PVM/Ms are essential for maintaining cochlear vascular architecture and stability.
AB - Tight control over cochlear blood flow (CoBF) and the blood-labyrinth barrier (BLB) in the striavascularis is critical for maintaining the ionic, fluid and energy balance necessary for hearing function. Inefficient CoBF and disruption of BLB integrity have long been considered major etiologic factors in a variety of hearing disorders. In this study, we investigate structural changes in the BLB of the striavascularis in age-graded C57BL/6 mice (1 to 21 months) with a focus on changes in two blood barrier accessory cells, namely pericytes (PCs) and perivascular-resident macrophage-like melanocytes (PVM/Ms). Decreased capillary density was detectable at 6 months, with significant capillary degeneration seen in 9- to 21-month-old mice. Reduced capillary density was highly correlated with lower numbers of PCs and PVM/Ms. “Drop-out” of PCs and “activation” of PVM/Ms were seen at 6 months, with drastic changes being observed by 21 months. With newly established in vitro three-dimensional cell-based co-culture models, we demonstrate that PCs and PVM/Ms are essential for maintaining cochlear vascular architecture and stability.
KW - Confocal laser microscopy
KW - Mouse (C57BL/6J)
KW - Pericyte∙perivascular resident macrophage-like melanocytes
KW - Strial blood–labyrinth barrier
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U2 - 10.1007/s00441-015-2147-2
DO - 10.1007/s00441-015-2147-2
M3 - Article
C2 - 25740201
AN - SCOPUS:84940451445
VL - 361
SP - 685
EP - 696
JO - Zeitschrift für Zellforschung und mikroskopische Anatomie (Vienna, Austria : 1948)
JF - Zeitschrift für Zellforschung und mikroskopische Anatomie (Vienna, Austria : 1948)
SN - 0302-766X
IS - 3
ER -