Structural basis for the mechanics of the endothelial cell

Catherine Galbraith; Thomas Deerinck; Mark Ellisman; Richard Skalak; Shu Chien

Structural basis for the mechanics of the endothelial cell

Catherine Galbraith, Thomas Deerinck, Mark Ellisman, Richard Skalak, Shu Chien

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

In order to determine how the application of shear stress to cultured endothelial cell monolayers leads to cytoskeletal reorganization, it is necessary to have an accurate knowledge of the organization of the cytoskeletal proteins. We have performed triple labeling for actin, tubulin, and vimentin within cultured bovine aortic endothelial cells to determine the relative positions of these cytoskeletal components. This information, coupled with an analysis of the material properties of the microfilaments, microtubules, and intermediate filaments, suggests the hypothesis that the microtubules form the primary structure within the endothelial cell that resists the application of shear stress.

Original language	English (US)
Title of host publication	Advances in Bioengineering
Editors	John M. Tarbell
Publisher	Publ by ASME
Pages	357-359
Number of pages	3
ISBN (Print)	0791810313
State	Published - 1993
Externally published	Yes
Event	Proceedings of the 1993 ASME Winter Annual Meeting - New Orleans, LA, USA Duration: Nov 28 1993 → Dec 3 1993

Publication series

Name	American Society of Mechanical Engineers, Bioengineering Division (Publication) BED
Volume	26

Other

Other	Proceedings of the 1993 ASME Winter Annual Meeting
City	New Orleans, LA, USA
Period	11/28/93 → 12/3/93

ASJC Scopus subject areas

General Engineering

Cite this

Structural basis for the mechanics of the endothelial cell. / Galbraith, Catherine; Deerinck, Thomas; Ellisman, Mark et al.
Advances in Bioengineering. ed. / John M. Tarbell. Publ by ASME, 1993. p. 357-359 (American Society of Mechanical Engineers, Bioengineering Division (Publication) BED; Vol. 26).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Galbraith, C, Deerinck, T, Ellisman, M, Skalak, R & Chien, S 1993, Structural basis for the mechanics of the endothelial cell. in JM Tarbell (ed.), Advances in Bioengineering. American Society of Mechanical Engineers, Bioengineering Division (Publication) BED, vol. 26, Publ by ASME, pp. 357-359, Proceedings of the 1993 ASME Winter Annual Meeting, New Orleans, LA, USA, 11/28/93.

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N2 - In order to determine how the application of shear stress to cultured endothelial cell monolayers leads to cytoskeletal reorganization, it is necessary to have an accurate knowledge of the organization of the cytoskeletal proteins. We have performed triple labeling for actin, tubulin, and vimentin within cultured bovine aortic endothelial cells to determine the relative positions of these cytoskeletal components. This information, coupled with an analysis of the material properties of the microfilaments, microtubules, and intermediate filaments, suggests the hypothesis that the microtubules form the primary structure within the endothelial cell that resists the application of shear stress.

AB - In order to determine how the application of shear stress to cultured endothelial cell monolayers leads to cytoskeletal reorganization, it is necessary to have an accurate knowledge of the organization of the cytoskeletal proteins. We have performed triple labeling for actin, tubulin, and vimentin within cultured bovine aortic endothelial cells to determine the relative positions of these cytoskeletal components. This information, coupled with an analysis of the material properties of the microfilaments, microtubules, and intermediate filaments, suggests the hypothesis that the microtubules form the primary structure within the endothelial cell that resists the application of shear stress.

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T2 - Proceedings of the 1993 ASME Winter Annual Meeting

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ER -

Structural basis for the mechanics of the endothelial cell

Abstract

Publication series

Other

ASJC Scopus subject areas

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