TY - JOUR
T1 - Structural and cellular characterization of electrospun recombinant human tropoelastin biomaterials
AU - McKenna, Kathryn A.
AU - Gregory, Kenton W.
AU - Sarao, Rebecca C.
AU - Maslen, Cheryl L.
AU - Glanville, Robert W.
AU - Hinds, Monica T.
N1 - Funding Information:
We would like to thank Dr. Jack M. McCarthy for his assistance with SEM and TEM imaging, Dr. Brian Kim for his assistance with cross-linking modalities, and Dr. Sean Kirkpatrick for his assistance in evaluating fiber orientation. Tropoelastin was produced with the excellent technical assistance of Amy Jay, Cher Hawkey, and Rose Merten. This work was funded in part by the NIH R01-HL095474 and Department of the Army, Grant Nos. W81XWH-04-1-0841 and W81XWH-05-1-0586. This work does not necessarily reflect the policy of the government and no official endorsement should be inferred.
PY - 2012/8
Y1 - 2012/8
N2 - An off-the-shelf vascular graft biomaterial for vascular bypass surgeries is an unmet clinical need. The vascular biomaterial must support cell growth, be non-thrombogenic, minimize intimal hyperplasia, match the structural properties of native vessels, and allow for regeneration of arterial tissue. Electrospun recombinant human tropoelastin (rTE) as a medial component of a vascular graft scaffold was investigated in this study by evaluating its structural properties, as well as its ability to support primary smooth muscle cell adhesion and growth. rTE solutions of 9, 15, and 20 wt% were electrospun into sheets with average fiber diameters of 167 ± 32, 522 ± 67, and 735 ± 270 nm, and average pore sizes of 0.4 ± 0.1, 5.8 ± 4.3, and 4.9 ± 2.4 μm, respectively. Electrospun rTE fibers were cross-linked with disuccinimidyl suberate to produce an insoluble fibrous polymeric recombinant tropoelastin (prTE) biomaterial. Smooth muscle cells attached via integrin binding to the rTE coatings and proliferated on prTE biomaterials at a comparable rate to growth on prTE coated glass, glass alone, and tissue culture plastic. Electrospun tropoelastin demonstrated the cell compatibility and design flexibility required of a graft biomaterial for vascular applications.
AB - An off-the-shelf vascular graft biomaterial for vascular bypass surgeries is an unmet clinical need. The vascular biomaterial must support cell growth, be non-thrombogenic, minimize intimal hyperplasia, match the structural properties of native vessels, and allow for regeneration of arterial tissue. Electrospun recombinant human tropoelastin (rTE) as a medial component of a vascular graft scaffold was investigated in this study by evaluating its structural properties, as well as its ability to support primary smooth muscle cell adhesion and growth. rTE solutions of 9, 15, and 20 wt% were electrospun into sheets with average fiber diameters of 167 ± 32, 522 ± 67, and 735 ± 270 nm, and average pore sizes of 0.4 ± 0.1, 5.8 ± 4.3, and 4.9 ± 2.4 μm, respectively. Electrospun rTE fibers were cross-linked with disuccinimidyl suberate to produce an insoluble fibrous polymeric recombinant tropoelastin (prTE) biomaterial. Smooth muscle cells attached via integrin binding to the rTE coatings and proliferated on prTE biomaterials at a comparable rate to growth on prTE coated glass, glass alone, and tissue culture plastic. Electrospun tropoelastin demonstrated the cell compatibility and design flexibility required of a graft biomaterial for vascular applications.
KW - cell adhesion
KW - cell proliferation
KW - elastin
KW - tissue engineering
KW - vascular biomaterial
UR - http://www.scopus.com/inward/record.url?scp=84855947040&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84855947040&partnerID=8YFLogxK
U2 - 10.1177/0885328211399480
DO - 10.1177/0885328211399480
M3 - Article
C2 - 21586601
AN - SCOPUS:84855947040
SN - 0885-3282
VL - 27
SP - 219
EP - 230
JO - Journal of Biomaterials Applications
JF - Journal of Biomaterials Applications
IS - 2
ER -