Structural analysis of membrane-bound retrovirus capsid proteins

Eric Barklis, Jason McDermott, Stephan Wilkens, Eric Schabtach, M. F. Schmid, Stephen Fuller, Sonya Karanjia, Zachary Love, Russell Jones, Yuanjui Rui, Xiumin Zhao, David Thompson

Research output: Contribution to journalArticle

87 Citations (Scopus)

Abstract

We have developed a system for analysis of histidine-tagged (His-tagged) retrovirus core (Gag) proteins, assembled in vitro on lipid monolayers consisting of egg phosphatidylcholine (PC) plus the novel lipid DHGN, DHGN was shown to chelate nickel by atomic absorption spectrometry, and DHGN-containing monolayers specifically bound gold conjugates of His-tagged proteins. Using PC+DHGN monolayers, we examined membrane-bound arrays of an N-terminal His-tagged Moloney murine leukemia virus (M-MuLV) capsid (CA) protein, His-MoCA, and in vivo studies suggest that in vitro-derived His-MoCA arrays reflect some of the Gag protein interactions which occur in assembling virus particles. The His-MoCA proteins formed extensive two-dimensional (2D) protein crystals, with reflections out to 9.5 Å resolution. The image-analyzed 2D projection of His-MoCA arrays revealed a distinct cage-like network. The asymmetry of the individual building blocks of the network led to the formation of two types of hexamer rings, surrounding protein-free cage holes. These results predict that Gag hexamers constitute a retrovirus core substructure, and that cage hole sizes define an exclusion limit for entry of retrovirus envelope proteins, or other plasma membrane proteins, into virus particles. We believe that the 2D crystallization method will permit the detailed analysis of retroviral Gag proteins and other His-tagged proteins.

Original languageEnglish (US)
Pages (from-to)1199-1213
Number of pages15
JournalEMBO Journal
Volume16
Issue number6
DOIs
StatePublished - Mar 17 1997

Fingerprint

Retroviridae Proteins
Capsid Proteins
Structural analysis
gag Gene Products
Histidine
Membranes
Viruses
Monolayers
Retroviridae
Proteins
Phosphatidylcholines
Virion
Moloney murine leukemia virus
Lipids
Atomic absorption spectrometry
Systems Analysis
Crystallization
Nickel
Gold
Ovum

Keywords

  • Electron microscopy
  • Image analysis
  • Lipid monolayer
  • Retrovirus

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Barklis, E., McDermott, J., Wilkens, S., Schabtach, E., Schmid, M. F., Fuller, S., ... Thompson, D. (1997). Structural analysis of membrane-bound retrovirus capsid proteins. EMBO Journal, 16(6), 1199-1213. https://doi.org/10.1093/emboj/16.6.1199

Structural analysis of membrane-bound retrovirus capsid proteins. / Barklis, Eric; McDermott, Jason; Wilkens, Stephan; Schabtach, Eric; Schmid, M. F.; Fuller, Stephen; Karanjia, Sonya; Love, Zachary; Jones, Russell; Rui, Yuanjui; Zhao, Xiumin; Thompson, David.

In: EMBO Journal, Vol. 16, No. 6, 17.03.1997, p. 1199-1213.

Research output: Contribution to journalArticle

Barklis, E, McDermott, J, Wilkens, S, Schabtach, E, Schmid, MF, Fuller, S, Karanjia, S, Love, Z, Jones, R, Rui, Y, Zhao, X & Thompson, D 1997, 'Structural analysis of membrane-bound retrovirus capsid proteins', EMBO Journal, vol. 16, no. 6, pp. 1199-1213. https://doi.org/10.1093/emboj/16.6.1199
Barklis E, McDermott J, Wilkens S, Schabtach E, Schmid MF, Fuller S et al. Structural analysis of membrane-bound retrovirus capsid proteins. EMBO Journal. 1997 Mar 17;16(6):1199-1213. https://doi.org/10.1093/emboj/16.6.1199
Barklis, Eric ; McDermott, Jason ; Wilkens, Stephan ; Schabtach, Eric ; Schmid, M. F. ; Fuller, Stephen ; Karanjia, Sonya ; Love, Zachary ; Jones, Russell ; Rui, Yuanjui ; Zhao, Xiumin ; Thompson, David. / Structural analysis of membrane-bound retrovirus capsid proteins. In: EMBO Journal. 1997 ; Vol. 16, No. 6. pp. 1199-1213.
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