Structural alterations in a type IV pilus subunit protein result in concurrent defects in multicellular behaviour and adherence to host tissue

Haesun Park, Matthew Wolfgang, Jos P M Van Putten, David Dorward, Stanley F. Hayes, Michael Koomey

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The ability of bacteria to establish complex communities on surfaces is believed to require both bacterial-substratum and bacterial-bacterial interactions, and type IV pili appear to play a critical but incompletely defined role in both these processes. Using the human pathogen Neisseria gonorrhoeae, spontaneous mutants defective in bacterial self-aggregative behaviour but quantitatively unaltered in pilus fibre expression were isolated by a unique selective scheme. The mutants, carrying single amino acid substitutions within the conserved amino-terminal domain of the pilus fibre subunit, were reduced in the ability to adhere to a human epithelial cell line. Co-expression of the altered alleles in the context of a wild-type pilE gene confirmed that they were dominant negative with respect to aggregation and human cell adherence. Strains expressing two copies of the altered alleles produced twice as much purifiable pili but retained the aggregative and adherence defects. Finally, the defects in aggregative behaviour and adherence of each of the mutants were suppressed by a loss-of-function mutation in the twitching motility gene pilT. The correlations between self-aggregation and the net capacity of the microbial population to adhere efficiently demonstrates the potential significance of bacterial cell-cell interactions to colonization.

Original languageEnglish (US)
Pages (from-to)293-307
Number of pages15
JournalMolecular Microbiology
Volume42
Issue number2
DOIs
StatePublished - 2001
Externally publishedYes

Fingerprint

Protein Subunits
Aptitude
Alleles
Cell Aggregation
Neisseria gonorrhoeae
Amino Acid Substitution
Cell Communication
Genes
Epithelial Cells
Bacteria
Cell Line
Mutation
Population

ASJC Scopus subject areas

  • Molecular Biology
  • Microbiology

Cite this

Structural alterations in a type IV pilus subunit protein result in concurrent defects in multicellular behaviour and adherence to host tissue. / Park, Haesun; Wolfgang, Matthew; Van Putten, Jos P M; Dorward, David; Hayes, Stanley F.; Koomey, Michael.

In: Molecular Microbiology, Vol. 42, No. 2, 2001, p. 293-307.

Research output: Contribution to journalArticle

Park, Haesun ; Wolfgang, Matthew ; Van Putten, Jos P M ; Dorward, David ; Hayes, Stanley F. ; Koomey, Michael. / Structural alterations in a type IV pilus subunit protein result in concurrent defects in multicellular behaviour and adherence to host tissue. In: Molecular Microbiology. 2001 ; Vol. 42, No. 2. pp. 293-307.
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