Stress-induced peptide release from rat intermediate pituitary - An ultrastructural analysis

James A. Carr, Linda C. Saland, Ann Samora, Sima Desai, Salvador Benevidez

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

We tested the hypothesis that acute restraint stress results in ultrastructural evidence for enhanced release of alpha-melanocyte-stimulating hormone (α-MSH) and β-endorphin from the intermediate lobe (IL) of the rat pituitary. Measurements of plasma α-MSH-and β-endorphin-immunoreactivity (ir) were used to confirm ultrastructural findings. Plasma α-MSH-ir was elevated after 20 and 30 min of restraint while plasma β-endorphin-ir peaked 10 min after the onset of restraint. Ultrastructural analysis revealed a decrease in the content of secretory granules within IL cells of stressed rats. Analysis of Golgi-related immature secretory granules in IL cells indicated that new peptide synthesis was not enhanced after 30 min of restraint. These results confirm previous studies showing and elevation of plasma β-endorphin and α-MSH-ir during acute restraint. Furthermore, these results indicate that quantitative analysis at the ultrastructural level can be used to assess peptide release from IL secretory cells during stress.

Original languageEnglish (US)
Pages (from-to)589-593
Number of pages5
JournalCell and Tissue Research
Volume261
Issue number3
DOIs
StatePublished - Sep 1990
Externally publishedYes

Fingerprint

Endorphins
Melanocyte-Stimulating Hormones
Rats
Plasmas
Peptides
Secretory Vesicles
Intermediate Pituitary Gland
alpha-MSH
Chemical analysis

Keywords

  • α-MSH
  • β-Endorphin
  • pars intermedia
  • Pituitary gland
  • Rat (Sprague-Dawley)
  • Secretory granules
  • Stress

ASJC Scopus subject areas

  • Anatomy
  • Clinical Biochemistry
  • Cell Biology

Cite this

Stress-induced peptide release from rat intermediate pituitary - An ultrastructural analysis. / Carr, James A.; Saland, Linda C.; Samora, Ann; Desai, Sima; Benevidez, Salvador.

In: Cell and Tissue Research, Vol. 261, No. 3, 09.1990, p. 589-593.

Research output: Contribution to journalArticle

Carr, James A. ; Saland, Linda C. ; Samora, Ann ; Desai, Sima ; Benevidez, Salvador. / Stress-induced peptide release from rat intermediate pituitary - An ultrastructural analysis. In: Cell and Tissue Research. 1990 ; Vol. 261, No. 3. pp. 589-593.
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