Streptavidin suppresses T cell activation and inhibits IL-2 production and CD25 expression

Kentaro Yomogida, Yuan Chou, Jonathan Pang, Bobby Baravati, Brian J. Maniaci, Shili Wu, Yong Zhu, Cong-Qiu Chu

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Streptavidin is widely used as a detection tool in biology research because of its high affinity and specificity binding to biotin. Biotin-streptavidin system has also been explored for detection of infection and tumor in clinical medicine. Here, we show immunosuppressive property of streptavidin on T cell activation and proliferation. Upon CD3 and CD28 stimulation, CD4 + T cells produce interleukin 2 (IL-2) and express IL-2 receptor α chain (CD25). Addition of streptavidin in T cell culture suppressed IL-2 synthesis and CD25 expression with no cytotoxicity. The immunosuppressive effect of streptavidin was reversed by excessive biotin. Conjugated to a single chain anti-CD7 variable fragment (scFvCD7), streptavidin was directly delivered to T cells and showed substantially more profound suppressive effect on T cell activation. These results suggest that streptavidin could potentially be used as a novel immunomodulator.

Original languageEnglish (US)
Pages (from-to)431-436
Number of pages6
JournalCytokine
Volume58
Issue number3
DOIs
StatePublished - Jun 2012

Fingerprint

Streptavidin
T-cells
Interleukin-2
Chemical activation
T-Lymphocytes
Biotin
Immunosuppressive Agents
Interleukin-2 Receptors
Clinical Medicine
Immunologic Factors
Cytotoxicity
Cell culture
Medicine
Tumors
Cell Culture Techniques
Cell Proliferation
Infection
Research
Neoplasms

Keywords

  • Biotin
  • Interleukin 2
  • Interleukin 2 receptor
  • Streptavidin
  • T Cell activation

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Hematology
  • Biochemistry
  • Molecular Biology

Cite this

Yomogida, K., Chou, Y., Pang, J., Baravati, B., Maniaci, B. J., Wu, S., ... Chu, C-Q. (2012). Streptavidin suppresses T cell activation and inhibits IL-2 production and CD25 expression. Cytokine, 58(3), 431-436. https://doi.org/10.1016/j.cyto.2012.02.007

Streptavidin suppresses T cell activation and inhibits IL-2 production and CD25 expression. / Yomogida, Kentaro; Chou, Yuan; Pang, Jonathan; Baravati, Bobby; Maniaci, Brian J.; Wu, Shili; Zhu, Yong; Chu, Cong-Qiu.

In: Cytokine, Vol. 58, No. 3, 06.2012, p. 431-436.

Research output: Contribution to journalArticle

Yomogida, K, Chou, Y, Pang, J, Baravati, B, Maniaci, BJ, Wu, S, Zhu, Y & Chu, C-Q 2012, 'Streptavidin suppresses T cell activation and inhibits IL-2 production and CD25 expression', Cytokine, vol. 58, no. 3, pp. 431-436. https://doi.org/10.1016/j.cyto.2012.02.007
Yomogida, Kentaro ; Chou, Yuan ; Pang, Jonathan ; Baravati, Bobby ; Maniaci, Brian J. ; Wu, Shili ; Zhu, Yong ; Chu, Cong-Qiu. / Streptavidin suppresses T cell activation and inhibits IL-2 production and CD25 expression. In: Cytokine. 2012 ; Vol. 58, No. 3. pp. 431-436.
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