Streptavidin suppresses T cell activation and inhibits IL-2 production and CD25 expression

Kentaro Yomogida, Yuan Chou, Jonathan Pang, Bobby Baravati, Brian J. Maniaci, Shili Wu, Yong Zhu, Cong Qiu Chu

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Streptavidin is widely used as a detection tool in biology research because of its high affinity and specificity binding to biotin. Biotin-streptavidin system has also been explored for detection of infection and tumor in clinical medicine. Here, we show immunosuppressive property of streptavidin on T cell activation and proliferation. Upon CD3 and CD28 stimulation, CD4 + T cells produce interleukin 2 (IL-2) and express IL-2 receptor α chain (CD25). Addition of streptavidin in T cell culture suppressed IL-2 synthesis and CD25 expression with no cytotoxicity. The immunosuppressive effect of streptavidin was reversed by excessive biotin. Conjugated to a single chain anti-CD7 variable fragment (scFvCD7), streptavidin was directly delivered to T cells and showed substantially more profound suppressive effect on T cell activation. These results suggest that streptavidin could potentially be used as a novel immunomodulator.

Original languageEnglish (US)
Pages (from-to)431-436
Number of pages6
JournalCytokine
Volume58
Issue number3
DOIs
StatePublished - Jun 1 2012

Keywords

  • Biotin
  • Interleukin 2
  • Interleukin 2 receptor
  • Streptavidin
  • T Cell activation

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Biochemistry
  • Hematology
  • Molecular Biology

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    Yomogida, K., Chou, Y., Pang, J., Baravati, B., Maniaci, B. J., Wu, S., Zhu, Y., & Chu, C. Q. (2012). Streptavidin suppresses T cell activation and inhibits IL-2 production and CD25 expression. Cytokine, 58(3), 431-436. https://doi.org/10.1016/j.cyto.2012.02.007