Strain differences in urocortin expression in the Edinger - Westphal nucleus and its relation to alcohol-induced hypothermia

R. K. Bachtell, N. O. Tsivkovskaia, Andrey Ryabinin

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

The Edinger - Westphal nucleus is the primary source of urocortin in rodent brain. Mapping of inducible transcription factors has shown that the Edinger - Westphal nucleus is preferentially sensitive to ethanol self-administration. In the present study we have immunohistochemically compared expression of urocortin and c-Fos in naive and ethanol-treated C57BL/6J and DBA/2J mouse inbred strains. We found that C57BL/6J mice possess significantly higher numbers of urocortin-expressing cells in the Edinger - Westphal compared to DBA/2J mice. Subsequent histological analysis confirmed a lower number of large neurons in the DBA/2J Edinger - Westphal nucleus. Surprisingly, despite the differences in structure, no strain differences were observed in the number of c-Fos-containing cells after acute (0.6-4.8 g/kg, i.p.) and repeated (2.4 g/kg, 14 days, one injection/day) administration of ethanol. Double-label immunohistochemistry showed that ethanol-induced c-Fos expression is present in different sets of Edinger - Westphal cells between the strains. Specifically, expression of c-Fos in C57BL/6J mice is preferentially induced in urocortin cells, while c-Fos in DBA/2J mice occurs in a mixed population of cells. Behavioral analysis of the B6D2 F2 intercross, a heterogeneous mouse strain, showed that the number of urocortin cells is positively correlated with basal temperatures and ethanol-induced hypothermia. Involvement of the Edinger - Westphal in alcohol-induced hypothermia is further confirmed by analysis of urocortin cells in the HOT/COLD selected lines. These results provide evidence that C57BL/6J and DBA/2J mice have structural differences in the Edinger - Westphal that can result in activation of different populations of neurons upon alcohol intoxication contributing to differential thermoregulation between these inbred strains.

Original languageEnglish (US)
Pages (from-to)421-434
Number of pages14
JournalNeuroscience
Volume113
Issue number2
DOIs
StatePublished - Aug 12 2002

Fingerprint

Urocortins
Induced Hypothermia
Inbred DBA Mouse
Alcohols
Ethanol
Inbred C57BL Mouse
Neurons
Alcoholic Intoxication
Inbred Strains Mice
Self Administration
Body Temperature Regulation
Population
Edinger-Westphal Nucleus
Rodentia
Transcription Factors
Cell Count
Immunohistochemistry
Injections
Temperature
Brain

Keywords

  • c-Fos
  • C57BL/6
  • DBA/2
  • HOT/COLD
  • Inbred strains
  • Selected lines

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Strain differences in urocortin expression in the Edinger - Westphal nucleus and its relation to alcohol-induced hypothermia. / Bachtell, R. K.; Tsivkovskaia, N. O.; Ryabinin, Andrey.

In: Neuroscience, Vol. 113, No. 2, 12.08.2002, p. 421-434.

Research output: Contribution to journalArticle

@article{17b5cd98ad45408c90620259d4da1fb3,
title = "Strain differences in urocortin expression in the Edinger - Westphal nucleus and its relation to alcohol-induced hypothermia",
abstract = "The Edinger - Westphal nucleus is the primary source of urocortin in rodent brain. Mapping of inducible transcription factors has shown that the Edinger - Westphal nucleus is preferentially sensitive to ethanol self-administration. In the present study we have immunohistochemically compared expression of urocortin and c-Fos in naive and ethanol-treated C57BL/6J and DBA/2J mouse inbred strains. We found that C57BL/6J mice possess significantly higher numbers of urocortin-expressing cells in the Edinger - Westphal compared to DBA/2J mice. Subsequent histological analysis confirmed a lower number of large neurons in the DBA/2J Edinger - Westphal nucleus. Surprisingly, despite the differences in structure, no strain differences were observed in the number of c-Fos-containing cells after acute (0.6-4.8 g/kg, i.p.) and repeated (2.4 g/kg, 14 days, one injection/day) administration of ethanol. Double-label immunohistochemistry showed that ethanol-induced c-Fos expression is present in different sets of Edinger - Westphal cells between the strains. Specifically, expression of c-Fos in C57BL/6J mice is preferentially induced in urocortin cells, while c-Fos in DBA/2J mice occurs in a mixed population of cells. Behavioral analysis of the B6D2 F2 intercross, a heterogeneous mouse strain, showed that the number of urocortin cells is positively correlated with basal temperatures and ethanol-induced hypothermia. Involvement of the Edinger - Westphal in alcohol-induced hypothermia is further confirmed by analysis of urocortin cells in the HOT/COLD selected lines. These results provide evidence that C57BL/6J and DBA/2J mice have structural differences in the Edinger - Westphal that can result in activation of different populations of neurons upon alcohol intoxication contributing to differential thermoregulation between these inbred strains.",
keywords = "c-Fos, C57BL/6, DBA/2, HOT/COLD, Inbred strains, Selected lines",
author = "Bachtell, {R. K.} and Tsivkovskaia, {N. O.} and Andrey Ryabinin",
year = "2002",
month = "8",
day = "12",
doi = "10.1016/S0306-4522(02)00174-4",
language = "English (US)",
volume = "113",
pages = "421--434",
journal = "Neuroscience",
issn = "0306-4522",
publisher = "Elsevier Limited",
number = "2",

}

TY - JOUR

T1 - Strain differences in urocortin expression in the Edinger - Westphal nucleus and its relation to alcohol-induced hypothermia

AU - Bachtell, R. K.

AU - Tsivkovskaia, N. O.

AU - Ryabinin, Andrey

PY - 2002/8/12

Y1 - 2002/8/12

N2 - The Edinger - Westphal nucleus is the primary source of urocortin in rodent brain. Mapping of inducible transcription factors has shown that the Edinger - Westphal nucleus is preferentially sensitive to ethanol self-administration. In the present study we have immunohistochemically compared expression of urocortin and c-Fos in naive and ethanol-treated C57BL/6J and DBA/2J mouse inbred strains. We found that C57BL/6J mice possess significantly higher numbers of urocortin-expressing cells in the Edinger - Westphal compared to DBA/2J mice. Subsequent histological analysis confirmed a lower number of large neurons in the DBA/2J Edinger - Westphal nucleus. Surprisingly, despite the differences in structure, no strain differences were observed in the number of c-Fos-containing cells after acute (0.6-4.8 g/kg, i.p.) and repeated (2.4 g/kg, 14 days, one injection/day) administration of ethanol. Double-label immunohistochemistry showed that ethanol-induced c-Fos expression is present in different sets of Edinger - Westphal cells between the strains. Specifically, expression of c-Fos in C57BL/6J mice is preferentially induced in urocortin cells, while c-Fos in DBA/2J mice occurs in a mixed population of cells. Behavioral analysis of the B6D2 F2 intercross, a heterogeneous mouse strain, showed that the number of urocortin cells is positively correlated with basal temperatures and ethanol-induced hypothermia. Involvement of the Edinger - Westphal in alcohol-induced hypothermia is further confirmed by analysis of urocortin cells in the HOT/COLD selected lines. These results provide evidence that C57BL/6J and DBA/2J mice have structural differences in the Edinger - Westphal that can result in activation of different populations of neurons upon alcohol intoxication contributing to differential thermoregulation between these inbred strains.

AB - The Edinger - Westphal nucleus is the primary source of urocortin in rodent brain. Mapping of inducible transcription factors has shown that the Edinger - Westphal nucleus is preferentially sensitive to ethanol self-administration. In the present study we have immunohistochemically compared expression of urocortin and c-Fos in naive and ethanol-treated C57BL/6J and DBA/2J mouse inbred strains. We found that C57BL/6J mice possess significantly higher numbers of urocortin-expressing cells in the Edinger - Westphal compared to DBA/2J mice. Subsequent histological analysis confirmed a lower number of large neurons in the DBA/2J Edinger - Westphal nucleus. Surprisingly, despite the differences in structure, no strain differences were observed in the number of c-Fos-containing cells after acute (0.6-4.8 g/kg, i.p.) and repeated (2.4 g/kg, 14 days, one injection/day) administration of ethanol. Double-label immunohistochemistry showed that ethanol-induced c-Fos expression is present in different sets of Edinger - Westphal cells between the strains. Specifically, expression of c-Fos in C57BL/6J mice is preferentially induced in urocortin cells, while c-Fos in DBA/2J mice occurs in a mixed population of cells. Behavioral analysis of the B6D2 F2 intercross, a heterogeneous mouse strain, showed that the number of urocortin cells is positively correlated with basal temperatures and ethanol-induced hypothermia. Involvement of the Edinger - Westphal in alcohol-induced hypothermia is further confirmed by analysis of urocortin cells in the HOT/COLD selected lines. These results provide evidence that C57BL/6J and DBA/2J mice have structural differences in the Edinger - Westphal that can result in activation of different populations of neurons upon alcohol intoxication contributing to differential thermoregulation between these inbred strains.

KW - c-Fos

KW - C57BL/6

KW - DBA/2

KW - HOT/COLD

KW - Inbred strains

KW - Selected lines

UR - http://www.scopus.com/inward/record.url?scp=0037067989&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037067989&partnerID=8YFLogxK

U2 - 10.1016/S0306-4522(02)00174-4

DO - 10.1016/S0306-4522(02)00174-4

M3 - Article

C2 - 12127099

AN - SCOPUS:0037067989

VL - 113

SP - 421

EP - 434

JO - Neuroscience

JF - Neuroscience

SN - 0306-4522

IS - 2

ER -