Strain dependent channel activation in cultured neurons

T. A. Lusardi; D. F. Meaney

Strain dependent channel activation in cultured neurons

T. A. Lusardi, D. F. Meaney

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

To determine the cellular response to mechanical strain, we have developed an in vitro device that allows us to monitor changes in individual cells under a microscope prior to and following a mechanical stretch. Previous data showed the rise in cytosolic calcium caused by a single mechanical stretch falls into three groups based on the applied strata and onset rate of strain. In the current study, we have treated NTera-2N neuronal cells with MK-801 (an NMDA channel blocker) to determine the contribution of NMDA channels to the intracellular calcium transient seen following mechanical stretch with a 205 ms onset time. Data shows that the calcium response is attenuated only at high strains, showing that the NMDA receptor is a primary mechanism of Ca⁺⁺ influx at this stimulation level.

Original language	English (US)
Title of host publication	Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
Publisher	IEEE
Pages	36
Number of pages	1
ISBN (Print)	0780356756
State	Published - 1999
Externally published	Yes
Event	Proceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS) - Atlanta, GA, USA Duration: Oct 13 1999 → Oct 16 1999

Publication series

Name	Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
Volume	1
ISSN (Print)	0589-1019

Other

Other	Proceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS)
City	Atlanta, GA, USA
Period	10/13/99 → 10/16/99

ASJC Scopus subject areas

Signal Processing
Biomedical Engineering
Computer Vision and Pattern Recognition
Health Informatics

Cite this

Strain dependent channel activation in cultured neurons. / Lusardi, T. A.; Meaney, D. F.
Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. IEEE, 1999. p. 36 (Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings; Vol. 1).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Lusardi, TA & Meaney, DF 1999, Strain dependent channel activation in cultured neurons. in Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings, vol. 1, IEEE, pp. 36, Proceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS), Atlanta, GA, USA, 10/13/99.

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abstract = "To determine the cellular response to mechanical strain, we have developed an in vitro device that allows us to monitor changes in individual cells under a microscope prior to and following a mechanical stretch. Previous data showed the rise in cytosolic calcium caused by a single mechanical stretch falls into three groups based on the applied strata and onset rate of strain. In the current study, we have treated NTera-2N neuronal cells with MK-801 (an NMDA channel blocker) to determine the contribution of NMDA channels to the intracellular calcium transient seen following mechanical stretch with a 205 ms onset time. Data shows that the calcium response is attenuated only at high strains, showing that the NMDA receptor is a primary mechanism of Ca++ influx at this stimulation level.",

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AB - To determine the cellular response to mechanical strain, we have developed an in vitro device that allows us to monitor changes in individual cells under a microscope prior to and following a mechanical stretch. Previous data showed the rise in cytosolic calcium caused by a single mechanical stretch falls into three groups based on the applied strata and onset rate of strain. In the current study, we have treated NTera-2N neuronal cells with MK-801 (an NMDA channel blocker) to determine the contribution of NMDA channels to the intracellular calcium transient seen following mechanical stretch with a 205 ms onset time. Data shows that the calcium response is attenuated only at high strains, showing that the NMDA receptor is a primary mechanism of Ca++ influx at this stimulation level.

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ER -

Strain dependent channel activation in cultured neurons

Abstract

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