Strain dependent channel activation in cultured neurons

T. A. Lusardi, D. F. Meaney

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

To determine the cellular response to mechanical strain, we have developed an in vitro device that allows us to monitor changes in individual cells under a microscope prior to and following a mechanical stretch. Previous data showed the rise in cytosolic calcium caused by a single mechanical stretch falls into three groups based on the applied strata and onset rate of strain. In the current study, we have treated NTera-2N neuronal cells with MK-801 (an NMDA channel blocker) to determine the contribution of NMDA channels to the intracellular calcium transient seen following mechanical stretch with a 205 ms onset time. Data shows that the calcium response is attenuated only at high strains, showing that the NMDA receptor is a primary mechanism of Ca++ influx at this stimulation level.

Original languageEnglish (US)
Title of host publicationAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
PublisherIEEE
Pages36
Number of pages1
ISBN (Print)0780356756
StatePublished - 1999
Externally publishedYes
EventProceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS) - Atlanta, GA, USA
Duration: Oct 13 1999Oct 16 1999

Publication series

NameAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
Volume1
ISSN (Print)0589-1019

Other

OtherProceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS)
CityAtlanta, GA, USA
Period10/13/9910/16/99

ASJC Scopus subject areas

  • Signal Processing
  • Biomedical Engineering
  • Computer Vision and Pattern Recognition
  • Health Informatics

Fingerprint Dive into the research topics of 'Strain dependent channel activation in cultured neurons'. Together they form a unique fingerprint.

Cite this