Stoichiometry-dependent formation of quantum dot-antibody bioconjugates: A complementary atomic force microscopy and agarose gel electrophoresis study

Barrett J. Nehilla, Tania Q. Vu, Tejal A. Desai

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

The unique advantages of quantum dot (QD) bioconjugates have motivated their application in biological assays. However, physical characterization of bioconjugated QDs after surface modification has often been overlooked. Here, biotinylated antibodies (biotin-IgG) were attached to commercial streptavidin-conjugated quantum dots (strep-QDs) at different stoichiometric ratios, and these QD bioconjugates were characterized with atomic force microscopy and discontinuous sodium dodecyl sulfate agarose gel electrophoresis (SDS-AGE). The results from these complementary analytical techniques showed that the molar ratio determined the relative sizes, molecular weights and morphologies of the QD bioconjugates. Additionally, the novel discontinuous SDS-AGE analysis confirmed specific binding between biotin-IgG and strep-QDs. Researchers who design QD bioconjugates for cell-based assays should consider stoichiometry-dependent differences in the physical properties of their QD bioconjugates.

Original languageEnglish (US)
Pages (from-to)20724-20730
Number of pages7
JournalJournal of Physical Chemistry B
Volume109
Issue number44
DOIs
StatePublished - Nov 10 2005

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Surfaces, Coatings and Films
  • Materials Chemistry

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