Stimulatory and inhibitory effects of prostaglandins on the gonadotropin-sensitive adenylate cyclase in the monkey corpus luteum

T. A. Molskness, C. A. VandeVoort, R. L. Stouffer

    Research output: Contribution to journalArticle

    49 Scopus citations

    Abstract

    Detailed analysis of the action of prostaglandins (PGs) on the corpus luteum in primate species is very limited. In this study we examined the response of the adenylate cyclase system to PGs in homogenates prepared from the corpus luteum of rhesus monkeys at midluteal phase of the menstrual cycle. The conversion of [α32P] ATP to [32P] cyclic AMP (cAMP) was assessed in the absence (control activity; 50 μM GTP) and presence of various concentrations of seven PGs and arachiodonic acid, either alone or in combination with 250 nM hCG. Cyclic AMP production increased up to three-fold in the presence of PGD2, PGE2, PGI2 or PGF; however PGA2, PGB2, 13,14-dihydro-15-keto PGE2 and arachidonic acid alone did not alter cAMP levels. In dose-response studies, adenylate cyclase was 10 and 100-fold more sensitive to PGD2 (Vmax at 1 × 10-5M) than to PGE2 or to PGI2 and PGF, respectively. Activity in the presence of hCG plus either PGD2, PGE2, PGI2 or PGF did not differ from that for hCG (or the PG) alone. In contrast, addition of PGA2 or arachidonate inhibited (p<0.05) hCG-stimulated cAMP production by 50 and 100 percent. We conclude that the gonadotropin-sensitive adenylate cyclase of the macaque corpus leteum is also modulated by several PGs. These factors may either mimic (e.g., PGD2, PGE2, PGI2) or suppress (PGA2)_ gonodotropin-stimulated cAMP production and possibly cAMP-mediated events in luteal cells.

    Original languageEnglish (US)
    Pages (from-to)279-290
    Number of pages12
    JournalProstaglandins
    Volume34
    Issue number2
    DOIs
    StatePublished - Aug 1987

    ASJC Scopus subject areas

    • Biochemistry
    • Endocrinology

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