Abstract
Detailed analysis of the action of prostaglandins (PGs) on the corpus luteum in primate species is very limited. In this study we examined the response of the adenylate cyclase system to PGs in homogenates prepared from the corpus luteum of rhesus monkeys at midluteal phase of the menstrual cycle. The conversion of [α32P] ATP to [32P] cyclic AMP (cAMP) was assessed in the absence (control activity; 50 μM GTP) and presence of various concentrations of seven PGs and arachiodonic acid, either alone or in combination with 250 nM hCG. Cyclic AMP production increased up to three-fold in the presence of PGD2, PGE2, PGI2 or PGF2α; however PGA2, PGB2, 13,14-dihydro-15-keto PGE2 and arachidonic acid alone did not alter cAMP levels. In dose-response studies, adenylate cyclase was 10 and 100-fold more sensitive to PGD2 (Vmax at 1 × 10-5M) than to PGE2 or to PGI2 and PGF2α, respectively. Activity in the presence of hCG plus either PGD2, PGE2, PGI2 or PGF2α did not differ from that for hCG (or the PG) alone. In contrast, addition of PGA2 or arachidonate inhibited (p2, PGE2, PGI2) or suppress (PGA2)_ gonodotropin-stimulated cAMP production and possibly cAMP-mediated events in luteal cells.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 279-290 |
| Number of pages | 12 |
| Journal | Prostaglandins |
| Volume | 34 |
| Issue number | 2 |
| DOIs | |
| State | Published - 1987 |
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ASJC Scopus subject areas
- Biochemistry
- Endocrinology
Cite this
Stimulatory and inhibitory effects of prostaglandins on the gonadotropin-sensitive adenylate cyclase in the monkey corpus luteum. / Molskness, T. A.; VandeVoort, C. A.; Stouffer, Richard.
In: Prostaglandins, Vol. 34, No. 2, 1987, p. 279-290.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Stimulatory and inhibitory effects of prostaglandins on the gonadotropin-sensitive adenylate cyclase in the monkey corpus luteum
AU - Molskness, T. A.
AU - VandeVoort, C. A.
AU - Stouffer, Richard
PY - 1987
Y1 - 1987
N2 - Detailed analysis of the action of prostaglandins (PGs) on the corpus luteum in primate species is very limited. In this study we examined the response of the adenylate cyclase system to PGs in homogenates prepared from the corpus luteum of rhesus monkeys at midluteal phase of the menstrual cycle. The conversion of [α32P] ATP to [32P] cyclic AMP (cAMP) was assessed in the absence (control activity; 50 μM GTP) and presence of various concentrations of seven PGs and arachiodonic acid, either alone or in combination with 250 nM hCG. Cyclic AMP production increased up to three-fold in the presence of PGD2, PGE2, PGI2 or PGF2α; however PGA2, PGB2, 13,14-dihydro-15-keto PGE2 and arachidonic acid alone did not alter cAMP levels. In dose-response studies, adenylate cyclase was 10 and 100-fold more sensitive to PGD2 (Vmax at 1 × 10-5M) than to PGE2 or to PGI2 and PGF2α, respectively. Activity in the presence of hCG plus either PGD2, PGE2, PGI2 or PGF2α did not differ from that for hCG (or the PG) alone. In contrast, addition of PGA2 or arachidonate inhibited (p2, PGE2, PGI2) or suppress (PGA2)_ gonodotropin-stimulated cAMP production and possibly cAMP-mediated events in luteal cells.
AB - Detailed analysis of the action of prostaglandins (PGs) on the corpus luteum in primate species is very limited. In this study we examined the response of the adenylate cyclase system to PGs in homogenates prepared from the corpus luteum of rhesus monkeys at midluteal phase of the menstrual cycle. The conversion of [α32P] ATP to [32P] cyclic AMP (cAMP) was assessed in the absence (control activity; 50 μM GTP) and presence of various concentrations of seven PGs and arachiodonic acid, either alone or in combination with 250 nM hCG. Cyclic AMP production increased up to three-fold in the presence of PGD2, PGE2, PGI2 or PGF2α; however PGA2, PGB2, 13,14-dihydro-15-keto PGE2 and arachidonic acid alone did not alter cAMP levels. In dose-response studies, adenylate cyclase was 10 and 100-fold more sensitive to PGD2 (Vmax at 1 × 10-5M) than to PGE2 or to PGI2 and PGF2α, respectively. Activity in the presence of hCG plus either PGD2, PGE2, PGI2 or PGF2α did not differ from that for hCG (or the PG) alone. In contrast, addition of PGA2 or arachidonate inhibited (p2, PGE2, PGI2) or suppress (PGA2)_ gonodotropin-stimulated cAMP production and possibly cAMP-mediated events in luteal cells.
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UR - http://www.scopus.com/inward/citedby.url?scp=0023574683&partnerID=8YFLogxK
U2 - 10.1016/0090-6980(87)90250-4
DO - 10.1016/0090-6980(87)90250-4
M3 - Article
C2 - 3671734
AN - SCOPUS:0023574683
VL - 34
SP - 279
EP - 290
JO - Prostaglandins and Other Lipid Mediators
JF - Prostaglandins and Other Lipid Mediators
SN - 1098-8823
IS - 2
ER -