Stimulatory and inhibitory effects of prostaglandins on the gonadotropin-sensitive adenylate cyclase in the monkey corpus luteum

T. A. Molskness, C. A. VandeVoort, Richard Stouffer

    Research output: Contribution to journalArticle

    49 Citations (Scopus)

    Abstract

    Detailed analysis of the action of prostaglandins (PGs) on the corpus luteum in primate species is very limited. In this study we examined the response of the adenylate cyclase system to PGs in homogenates prepared from the corpus luteum of rhesus monkeys at midluteal phase of the menstrual cycle. The conversion of [α32P] ATP to [32P] cyclic AMP (cAMP) was assessed in the absence (control activity; 50 μM GTP) and presence of various concentrations of seven PGs and arachiodonic acid, either alone or in combination with 250 nM hCG. Cyclic AMP production increased up to three-fold in the presence of PGD2, PGE2, PGI2 or PGF; however PGA2, PGB2, 13,14-dihydro-15-keto PGE2 and arachidonic acid alone did not alter cAMP levels. In dose-response studies, adenylate cyclase was 10 and 100-fold more sensitive to PGD2 (Vmax at 1 × 10-5M) than to PGE2 or to PGI2 and PGF, respectively. Activity in the presence of hCG plus either PGD2, PGE2, PGI2 or PGF did not differ from that for hCG (or the PG) alone. In contrast, addition of PGA2 or arachidonate inhibited (p2, PGE2, PGI2) or suppress (PGA2)_ gonodotropin-stimulated cAMP production and possibly cAMP-mediated events in luteal cells.

    Original languageEnglish (US)
    Pages (from-to)279-290
    Number of pages12
    JournalProstaglandins
    Volume34
    Issue number2
    DOIs
    StatePublished - 1987

    Fingerprint

    Corpus Luteum
    Gonadotropins
    Adenylyl Cyclases
    Cyclic AMP
    Prostaglandins
    Haplorhini
    Epoprostenol
    Dinoprostone
    Prostaglandin D2
    Dinoprost
    Luteal Cells
    Menstrual Cycle
    Guanosine Triphosphate
    Macaca mulatta
    Arachidonic Acid
    Primates
    Adenosine Triphosphate
    Acids
    prostaglandin A2

    ASJC Scopus subject areas

    • Biochemistry
    • Endocrinology

    Cite this

    Stimulatory and inhibitory effects of prostaglandins on the gonadotropin-sensitive adenylate cyclase in the monkey corpus luteum. / Molskness, T. A.; VandeVoort, C. A.; Stouffer, Richard.

    In: Prostaglandins, Vol. 34, No. 2, 1987, p. 279-290.

    Research output: Contribution to journalArticle

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