Stimulation of pp60(c-src) kinase activity in FDC-P1 cells by polyoma middle-T antigen and hematopoietic growth factors

J. Muser, Stefanie Kaech-Petrie, C. Moroni, K. Ballmer-Hofer

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Either of two hematopoietic growth factors, GM-CSF or Il-3, are required for the growth of the bone marrow-derived FDC-P1 cell line. These factors induce cellular tyrosine-specific protein kinases when added to resting cells. The receptors for these factors have not been unambiguously shown to contain a kinase domain. To determine whether src-related kinases, in particular pp60(C-src, are regulated by GM-CSF or IL-3, FDC-P1 cells were transfected with plasmids carrying polyoma middle-T antigen, a potent activator of pp60(c-src). Middle-T-expressing cells showed a reduced requirement for GM-CSF and IL-3 and selected clones grew in the absence of these factors. Middle-T formed a complex with pp60(c-src) and stimulated its in vitro kinase activity 20-50 fold. pp60(c-src) kinase activity was further increased if middle T-expressing, factor-independent cells were treated with GM-CSF or IL-3.

Original languageEnglish (US)
Pages (from-to)1433-1439
Number of pages7
JournalOncogene
Volume4
Issue number12
StatePublished - 1989
Externally publishedYes

Fingerprint

Proto-Oncogene Proteins pp60(c-src)
Viral Tumor Antigens
Granulocyte-Macrophage Colony-Stimulating Factor
Interleukin-3
Intercellular Signaling Peptides and Proteins
Phosphotransferases
src-Family Kinases
Protein-Tyrosine Kinases
Plasmids
Clone Cells
Bone Marrow
Cell Line
CSK tyrosine-protein kinase
Growth

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

Cite this

Stimulation of pp60(c-src) kinase activity in FDC-P1 cells by polyoma middle-T antigen and hematopoietic growth factors. / Muser, J.; Kaech-Petrie, Stefanie; Moroni, C.; Ballmer-Hofer, K.

In: Oncogene, Vol. 4, No. 12, 1989, p. 1433-1439.

Research output: Contribution to journalArticle

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