Abstract
Either of two hematopoietic growth factors, GM-CSF or Il-3, are required for the growth of the bone marrow-derived FDC-P1 cell line. These factors induce cellular tyrosine-specific protein kinases when added to resting cells. The receptors for these factors have not been unambiguously shown to contain a kinase domain. To determine whether src-related kinases, in particular pp60(C-src, are regulated by GM-CSF or IL-3, FDC-P1 cells were transfected with plasmids carrying polyoma middle-T antigen, a potent activator of pp60(c-src). Middle-T-expressing cells showed a reduced requirement for GM-CSF and IL-3 and selected clones grew in the absence of these factors. Middle-T formed a complex with pp60(c-src) and stimulated its in vitro kinase activity 20-50 fold. pp60(c-src) kinase activity was further increased if middle T-expressing, factor-independent cells were treated with GM-CSF or IL-3.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1433-1439 |
| Number of pages | 7 |
| Journal | Oncogene |
| Volume | 4 |
| Issue number | 12 |
| State | Published - 1989 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Molecular Biology
- Cancer Research
- Genetics
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Stimulation of pp60(c-src) kinase activity in FDC-P1 cells by polyoma middle-T antigen and hematopoietic growth factors. / Muser, J.; Kaech-Petrie, Stefanie; Moroni, C.; Ballmer-Hofer, K.
In: Oncogene, Vol. 4, No. 12, 1989, p. 1433-1439.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Stimulation of pp60(c-src) kinase activity in FDC-P1 cells by polyoma middle-T antigen and hematopoietic growth factors
AU - Muser, J.
AU - Kaech-Petrie, Stefanie
AU - Moroni, C.
AU - Ballmer-Hofer, K.
PY - 1989
Y1 - 1989
N2 - Either of two hematopoietic growth factors, GM-CSF or Il-3, are required for the growth of the bone marrow-derived FDC-P1 cell line. These factors induce cellular tyrosine-specific protein kinases when added to resting cells. The receptors for these factors have not been unambiguously shown to contain a kinase domain. To determine whether src-related kinases, in particular pp60(C-src, are regulated by GM-CSF or IL-3, FDC-P1 cells were transfected with plasmids carrying polyoma middle-T antigen, a potent activator of pp60(c-src). Middle-T-expressing cells showed a reduced requirement for GM-CSF and IL-3 and selected clones grew in the absence of these factors. Middle-T formed a complex with pp60(c-src) and stimulated its in vitro kinase activity 20-50 fold. pp60(c-src) kinase activity was further increased if middle T-expressing, factor-independent cells were treated with GM-CSF or IL-3.
AB - Either of two hematopoietic growth factors, GM-CSF or Il-3, are required for the growth of the bone marrow-derived FDC-P1 cell line. These factors induce cellular tyrosine-specific protein kinases when added to resting cells. The receptors for these factors have not been unambiguously shown to contain a kinase domain. To determine whether src-related kinases, in particular pp60(C-src, are regulated by GM-CSF or IL-3, FDC-P1 cells were transfected with plasmids carrying polyoma middle-T antigen, a potent activator of pp60(c-src). Middle-T-expressing cells showed a reduced requirement for GM-CSF and IL-3 and selected clones grew in the absence of these factors. Middle-T formed a complex with pp60(c-src) and stimulated its in vitro kinase activity 20-50 fold. pp60(c-src) kinase activity was further increased if middle T-expressing, factor-independent cells were treated with GM-CSF or IL-3.
UR - http://www.scopus.com/inward/record.url?scp=0024471507&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024471507&partnerID=8YFLogxK
M3 - Article
C2 - 2480561
AN - SCOPUS:0024471507
VL - 4
SP - 1433
EP - 1439
JO - Oncogene
JF - Oncogene
SN - 0950-9232
IS - 12
ER -