Stimulation of mouse melanocyte proliferation by Piper nigrum fruit extract and its main alkaloid, piperine

Zhixiu Lin, J. R S Hoult, Dorothy C. Bennett, Amala Soumyanath

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

During a herbal screening programme to find potential repigmenting agents for the treatment of vitiligo, Piper nigrum L. fruit (black pepper) extract was found to possess growth-stimulatory activity towards cultured melanocytes. Its aqueous extract at 0.1 mg/ml was observed to cause nearly 300% stimulation of the growth of a cultured mouse melanocyte line, melan-a, in 8 clays (p <0.01). Piperine (1-piperoylpiperidine), the main alkaloid from Piper nigrum fruit, also significantly stimulated melan-a cell growth. Both Piper nigrum extract and piperine induced morphological alterations in melana cells, with more and longer dendrites observed. The augmentation of growth by piperine was effectively inhibited by RO-318220, a selective protein kinase C (PKC) inhibitor, suggesting that PKC signalling is involved in its activity. This is the first full report on such an activity of black pepper and piperine.

Original languageEnglish (US)
Pages (from-to)600-603
Number of pages4
JournalPlanta Medica
Volume65
Issue number7
DOIs
StatePublished - 1999
Externally publishedYes

Fingerprint

piperine
Piper nigrum
fruit extracts
melanocytes
Melanocytes
Fruits
Alkaloids
alkaloids
black pepper
Fruit
protein kinase C
mice
extracts
Protein Kinase C
Growth
fruits
dendrites
MART-1 Antigen
cell growth
Cell growth

Keywords

  • Melan-a cell line
  • Piper nigrum
  • Piperaceae
  • Piperine
  • Proliferation
  • Vitiligo

ASJC Scopus subject areas

  • Plant Science
  • Drug Discovery
  • Organic Chemistry
  • Pharmacology

Cite this

Stimulation of mouse melanocyte proliferation by Piper nigrum fruit extract and its main alkaloid, piperine. / Lin, Zhixiu; Hoult, J. R S; Bennett, Dorothy C.; Soumyanath, Amala.

In: Planta Medica, Vol. 65, No. 7, 1999, p. 600-603.

Research output: Contribution to journalArticle

Lin, Zhixiu ; Hoult, J. R S ; Bennett, Dorothy C. ; Soumyanath, Amala. / Stimulation of mouse melanocyte proliferation by Piper nigrum fruit extract and its main alkaloid, piperine. In: Planta Medica. 1999 ; Vol. 65, No. 7. pp. 600-603.
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