Stimulation of d2 dopamine receptors decreases intracellular calcium levels in rat anterior pituitary cells but not striatal synaptosomes: A flow cytometric study using indo‐1

Marina Wolf, Gregory Kapatos

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19 Citations (Scopus)

Abstract

An important question is whether all D2 dopamine (DA) receptors employ the same signal transduction mechanisms. Anterior pituitary cells and striatal synaptosomes, which possess pharmacologically similar D2 DA receptors, were compared with respect to the effect of D2 DA receptor stimulation on free intracellular Ca2+ levels ([Ca2+]i). Flow cytometry, in combination with either the fluorescent calcium indicator indo‐1 or fluorescent voltage‐sensitive dyes, was used to measure [Ca2+]i and to detect changes in membrane potential. In subpopulations of anterior pituitary cells, increases in [Ca2+]i were produced by elevated K+, veratridine, thyrotropin‐releasing hormone, and BAY K 8644. These increases were blocked by nifedipine, suggesting the involvement of L‐type voltage‐sensitive calcium channels (VSCC's). In 10–15% of the cells, D2 agonists decreased resting [Ca2+]i, reversed stimulus‐induced increases in [Ca2+]i, and caused a hyperpolarization. In striatal synaptosomes, elevated K+ and veratridine also increased [Ca2+]i. However, the K+‐induced increase was eliminated if choline was substituted for Na+ in the medium, suggesting that Ca2+ entry in response to sustained K+ depolarization resulted from reversal of Na+/Ca2+ exchange. Nifedipine and verapamil inhibited K+‐induced increases in [Ca2+]i only at concentrations greater than 10 μM, while ω‐conotoxin had no effect. D2 agonists had no effect on resting or stimulated [Ca2+]i but did hyperpolarize 10–20% of the synaptosomes, indicating that D2 DA receptors are functional in this preparation. The ability of pituitary but not striatal D2 DA receptors to modulate [Ca2+]i may reflect the fact that the two systems differ with respect to pathways for Ca2+ influx.

Original languageEnglish (US)
Pages (from-to)353-370
Number of pages18
JournalSynapse
Volume4
Issue number4
DOIs
StatePublished - Jan 1 1989
Externally publishedYes

Fingerprint

Corpus Striatum
Dopamine D2 Receptors
Synaptosomes
Calcium
Veratridine
Nifedipine
Conotoxins
Calcium Channels
Verapamil
Choline
Fluorescent Dyes
Membrane Potentials
Signal Transduction
Flow Cytometry
Hormones

Keywords

  • Calcium
  • Flow cytometry
  • Lactotrophs
  • Voltage‐sensitive dyes

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

Cite this

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title = "Stimulation of d2 dopamine receptors decreases intracellular calcium levels in rat anterior pituitary cells but not striatal synaptosomes: A flow cytometric study using indo‐1",
abstract = "An important question is whether all D2 dopamine (DA) receptors employ the same signal transduction mechanisms. Anterior pituitary cells and striatal synaptosomes, which possess pharmacologically similar D2 DA receptors, were compared with respect to the effect of D2 DA receptor stimulation on free intracellular Ca2+ levels ([Ca2+]i). Flow cytometry, in combination with either the fluorescent calcium indicator indo‐1 or fluorescent voltage‐sensitive dyes, was used to measure [Ca2+]i and to detect changes in membrane potential. In subpopulations of anterior pituitary cells, increases in [Ca2+]i were produced by elevated K+, veratridine, thyrotropin‐releasing hormone, and BAY K 8644. These increases were blocked by nifedipine, suggesting the involvement of L‐type voltage‐sensitive calcium channels (VSCC's). In 10–15{\%} of the cells, D2 agonists decreased resting [Ca2+]i, reversed stimulus‐induced increases in [Ca2+]i, and caused a hyperpolarization. In striatal synaptosomes, elevated K+ and veratridine also increased [Ca2+]i. However, the K+‐induced increase was eliminated if choline was substituted for Na+ in the medium, suggesting that Ca2+ entry in response to sustained K+ depolarization resulted from reversal of Na+/Ca2+ exchange. Nifedipine and verapamil inhibited K+‐induced increases in [Ca2+]i only at concentrations greater than 10 μM, while ω‐conotoxin had no effect. D2 agonists had no effect on resting or stimulated [Ca2+]i but did hyperpolarize 10–20{\%} of the synaptosomes, indicating that D2 DA receptors are functional in this preparation. The ability of pituitary but not striatal D2 DA receptors to modulate [Ca2+]i may reflect the fact that the two systems differ with respect to pathways for Ca2+ influx.",
keywords = "Calcium, Flow cytometry, Lactotrophs, Voltage‐sensitive dyes",
author = "Marina Wolf and Gregory Kapatos",
year = "1989",
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volume = "4",
pages = "353--370",
journal = "Synapse",
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AU - Kapatos, Gregory

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N2 - An important question is whether all D2 dopamine (DA) receptors employ the same signal transduction mechanisms. Anterior pituitary cells and striatal synaptosomes, which possess pharmacologically similar D2 DA receptors, were compared with respect to the effect of D2 DA receptor stimulation on free intracellular Ca2+ levels ([Ca2+]i). Flow cytometry, in combination with either the fluorescent calcium indicator indo‐1 or fluorescent voltage‐sensitive dyes, was used to measure [Ca2+]i and to detect changes in membrane potential. In subpopulations of anterior pituitary cells, increases in [Ca2+]i were produced by elevated K+, veratridine, thyrotropin‐releasing hormone, and BAY K 8644. These increases were blocked by nifedipine, suggesting the involvement of L‐type voltage‐sensitive calcium channels (VSCC's). In 10–15% of the cells, D2 agonists decreased resting [Ca2+]i, reversed stimulus‐induced increases in [Ca2+]i, and caused a hyperpolarization. In striatal synaptosomes, elevated K+ and veratridine also increased [Ca2+]i. However, the K+‐induced increase was eliminated if choline was substituted for Na+ in the medium, suggesting that Ca2+ entry in response to sustained K+ depolarization resulted from reversal of Na+/Ca2+ exchange. Nifedipine and verapamil inhibited K+‐induced increases in [Ca2+]i only at concentrations greater than 10 μM, while ω‐conotoxin had no effect. D2 agonists had no effect on resting or stimulated [Ca2+]i but did hyperpolarize 10–20% of the synaptosomes, indicating that D2 DA receptors are functional in this preparation. The ability of pituitary but not striatal D2 DA receptors to modulate [Ca2+]i may reflect the fact that the two systems differ with respect to pathways for Ca2+ influx.

AB - An important question is whether all D2 dopamine (DA) receptors employ the same signal transduction mechanisms. Anterior pituitary cells and striatal synaptosomes, which possess pharmacologically similar D2 DA receptors, were compared with respect to the effect of D2 DA receptor stimulation on free intracellular Ca2+ levels ([Ca2+]i). Flow cytometry, in combination with either the fluorescent calcium indicator indo‐1 or fluorescent voltage‐sensitive dyes, was used to measure [Ca2+]i and to detect changes in membrane potential. In subpopulations of anterior pituitary cells, increases in [Ca2+]i were produced by elevated K+, veratridine, thyrotropin‐releasing hormone, and BAY K 8644. These increases were blocked by nifedipine, suggesting the involvement of L‐type voltage‐sensitive calcium channels (VSCC's). In 10–15% of the cells, D2 agonists decreased resting [Ca2+]i, reversed stimulus‐induced increases in [Ca2+]i, and caused a hyperpolarization. In striatal synaptosomes, elevated K+ and veratridine also increased [Ca2+]i. However, the K+‐induced increase was eliminated if choline was substituted for Na+ in the medium, suggesting that Ca2+ entry in response to sustained K+ depolarization resulted from reversal of Na+/Ca2+ exchange. Nifedipine and verapamil inhibited K+‐induced increases in [Ca2+]i only at concentrations greater than 10 μM, while ω‐conotoxin had no effect. D2 agonists had no effect on resting or stimulated [Ca2+]i but did hyperpolarize 10–20% of the synaptosomes, indicating that D2 DA receptors are functional in this preparation. The ability of pituitary but not striatal D2 DA receptors to modulate [Ca2+]i may reflect the fact that the two systems differ with respect to pathways for Ca2+ influx.

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