Stimulated exocytosis of endosomes in goldfish retinal bipolar neurons

Michael R. Coggins, Chad P. Grabner, Wolfhard Almers, David Zenisek

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

After exocytosis, synaptic vesicle components are selectively retrieved by clathrin-mediated endocytosis and then re-used in future rounds of transmitter release. Under some conditions, synaptic terminals in addition perform bulk endocytosis of large membranous sacs. Bulk endocytosis is less selective than clathrin-mediated endocytosis and probably internalizes components normally targeted to the plasma membrane. Nonetheless, this process plays a major role in some tonic ribbon-type synapses, which release neurotransmitter for prolonged periods of time. We show here, that large endosomes formed after strong and prolonged stimulation undergo stimulated exocytosis in retinal bipolar neurons. The result suggests how cells might return erroneously internalized components to the plasma membrane, and also demonstrates that synaptic vesicles are not the only neuronal organelle that stains with styryl dyes and undergoes stimulated exocytosis.

Original languageEnglish (US)
Pages (from-to)853-865
Number of pages13
JournalJournal of Physiology
Volume584
Issue number3
DOIs
StatePublished - Nov 1 2007

Fingerprint

Retinal Neurons
Goldfish
Endosomes
Exocytosis
Endocytosis
Clathrin
Synaptic Vesicles
Coloring Agents
Cell Membrane
Presynaptic Terminals
Organelles
Synapses
Neurotransmitter Agents

ASJC Scopus subject areas

  • Physiology

Cite this

Stimulated exocytosis of endosomes in goldfish retinal bipolar neurons. / Coggins, Michael R.; Grabner, Chad P.; Almers, Wolfhard; Zenisek, David.

In: Journal of Physiology, Vol. 584, No. 3, 01.11.2007, p. 853-865.

Research output: Contribution to journalArticle

Coggins, MR, Grabner, CP, Almers, W & Zenisek, D 2007, 'Stimulated exocytosis of endosomes in goldfish retinal bipolar neurons', Journal of Physiology, vol. 584, no. 3, pp. 853-865. https://doi.org/10.1113/jphysiol.2007.140848
Coggins, Michael R. ; Grabner, Chad P. ; Almers, Wolfhard ; Zenisek, David. / Stimulated exocytosis of endosomes in goldfish retinal bipolar neurons. In: Journal of Physiology. 2007 ; Vol. 584, No. 3. pp. 853-865.
@article{2b2911b3ddf04d3887d19f0304d7d409,
title = "Stimulated exocytosis of endosomes in goldfish retinal bipolar neurons",
abstract = "After exocytosis, synaptic vesicle components are selectively retrieved by clathrin-mediated endocytosis and then re-used in future rounds of transmitter release. Under some conditions, synaptic terminals in addition perform bulk endocytosis of large membranous sacs. Bulk endocytosis is less selective than clathrin-mediated endocytosis and probably internalizes components normally targeted to the plasma membrane. Nonetheless, this process plays a major role in some tonic ribbon-type synapses, which release neurotransmitter for prolonged periods of time. We show here, that large endosomes formed after strong and prolonged stimulation undergo stimulated exocytosis in retinal bipolar neurons. The result suggests how cells might return erroneously internalized components to the plasma membrane, and also demonstrates that synaptic vesicles are not the only neuronal organelle that stains with styryl dyes and undergoes stimulated exocytosis.",
author = "Coggins, {Michael R.} and Grabner, {Chad P.} and Wolfhard Almers and David Zenisek",
year = "2007",
month = "11",
day = "1",
doi = "10.1113/jphysiol.2007.140848",
language = "English (US)",
volume = "584",
pages = "853--865",
journal = "Journal of Physiology",
issn = "0022-3751",
publisher = "Wiley-Blackwell",
number = "3",

}

TY - JOUR

T1 - Stimulated exocytosis of endosomes in goldfish retinal bipolar neurons

AU - Coggins, Michael R.

AU - Grabner, Chad P.

AU - Almers, Wolfhard

AU - Zenisek, David

PY - 2007/11/1

Y1 - 2007/11/1

N2 - After exocytosis, synaptic vesicle components are selectively retrieved by clathrin-mediated endocytosis and then re-used in future rounds of transmitter release. Under some conditions, synaptic terminals in addition perform bulk endocytosis of large membranous sacs. Bulk endocytosis is less selective than clathrin-mediated endocytosis and probably internalizes components normally targeted to the plasma membrane. Nonetheless, this process plays a major role in some tonic ribbon-type synapses, which release neurotransmitter for prolonged periods of time. We show here, that large endosomes formed after strong and prolonged stimulation undergo stimulated exocytosis in retinal bipolar neurons. The result suggests how cells might return erroneously internalized components to the plasma membrane, and also demonstrates that synaptic vesicles are not the only neuronal organelle that stains with styryl dyes and undergoes stimulated exocytosis.

AB - After exocytosis, synaptic vesicle components are selectively retrieved by clathrin-mediated endocytosis and then re-used in future rounds of transmitter release. Under some conditions, synaptic terminals in addition perform bulk endocytosis of large membranous sacs. Bulk endocytosis is less selective than clathrin-mediated endocytosis and probably internalizes components normally targeted to the plasma membrane. Nonetheless, this process plays a major role in some tonic ribbon-type synapses, which release neurotransmitter for prolonged periods of time. We show here, that large endosomes formed after strong and prolonged stimulation undergo stimulated exocytosis in retinal bipolar neurons. The result suggests how cells might return erroneously internalized components to the plasma membrane, and also demonstrates that synaptic vesicles are not the only neuronal organelle that stains with styryl dyes and undergoes stimulated exocytosis.

UR - http://www.scopus.com/inward/record.url?scp=35649023145&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=35649023145&partnerID=8YFLogxK

U2 - 10.1113/jphysiol.2007.140848

DO - 10.1113/jphysiol.2007.140848

M3 - Article

C2 - 17823206

AN - SCOPUS:35649023145

VL - 584

SP - 853

EP - 865

JO - Journal of Physiology

JF - Journal of Physiology

SN - 0022-3751

IS - 3

ER -