TY - JOUR
T1 - Stimulated exocytosis of endosomes in goldfish retinal bipolar neurons
AU - Coggins, Michael R.
AU - Grabner, Chad P.
AU - Almers, Wolfhard
AU - Zenisek, David
PY - 2007/11/1
Y1 - 2007/11/1
N2 - After exocytosis, synaptic vesicle components are selectively retrieved by clathrin-mediated endocytosis and then re-used in future rounds of transmitter release. Under some conditions, synaptic terminals in addition perform bulk endocytosis of large membranous sacs. Bulk endocytosis is less selective than clathrin-mediated endocytosis and probably internalizes components normally targeted to the plasma membrane. Nonetheless, this process plays a major role in some tonic ribbon-type synapses, which release neurotransmitter for prolonged periods of time. We show here, that large endosomes formed after strong and prolonged stimulation undergo stimulated exocytosis in retinal bipolar neurons. The result suggests how cells might return erroneously internalized components to the plasma membrane, and also demonstrates that synaptic vesicles are not the only neuronal organelle that stains with styryl dyes and undergoes stimulated exocytosis.
AB - After exocytosis, synaptic vesicle components are selectively retrieved by clathrin-mediated endocytosis and then re-used in future rounds of transmitter release. Under some conditions, synaptic terminals in addition perform bulk endocytosis of large membranous sacs. Bulk endocytosis is less selective than clathrin-mediated endocytosis and probably internalizes components normally targeted to the plasma membrane. Nonetheless, this process plays a major role in some tonic ribbon-type synapses, which release neurotransmitter for prolonged periods of time. We show here, that large endosomes formed after strong and prolonged stimulation undergo stimulated exocytosis in retinal bipolar neurons. The result suggests how cells might return erroneously internalized components to the plasma membrane, and also demonstrates that synaptic vesicles are not the only neuronal organelle that stains with styryl dyes and undergoes stimulated exocytosis.
UR - http://www.scopus.com/inward/record.url?scp=35649023145&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=35649023145&partnerID=8YFLogxK
U2 - 10.1113/jphysiol.2007.140848
DO - 10.1113/jphysiol.2007.140848
M3 - Article
C2 - 17823206
AN - SCOPUS:35649023145
SN - 0022-3751
VL - 584
SP - 853
EP - 865
JO - Journal of Physiology
JF - Journal of Physiology
IS - 3
ER -