Steroid regulation of estrogen and progestin receptor messenger ribonucleic acid in monkey hypothalamus and pituitary

Cynthia Bethea, N. A. Brown, Steven Kohama

    Research output: Contribution to journalArticle

    68 Citations (Scopus)

    Abstract

    The regulation of estrogen and progestin receptor (ER and PR, respectively) messenger RNA (mRNA) and protein by their cognate hormones was examined in the hypothalamus and pituitary of steroid-treated monkeys. Rhesus macaques (Macaca mulatta) were ovariectomized, hysterectomized (spayed), and implanted with SILASTIC brand capsules containing 17β-estradiol (E) or progesterone (P). The spayed control group received empty capsules. The E- treated group received E-filled capsules for 28 days. The E + P-treated animals received an E-filled capsule for 28 days and then a P-filled capsule for the last 14 of the 28 days. Steroid regulation of ER and PR mRNA levels in the hypothalamus and pituitary was examined with in situ hybridization. In the hypothalamus, ER and PR immunodetectable proteins were also examined in nearby sections. In the pituitary, mRNA levels were compared to previous ER and PR protein analysis of identically treated animals. E treatment induced PR mRNA in the medial basal hypothalamus and pituitary. Supplemental P treatment had no effect on PR mRNA levels in the hypothalamus, but markedly reduced PR mRNA in the pituitary. There was excellent agreement with PR protein detection by immunocytochemistry. E treatment had no effect on ER mRNA in the hypothalamus or pituitary. Supplemental P treatment decreased ER mRNA in the ventromedial nucleus, but not in the arcuate nucleus or pituitary. There was agreement between ER mRNA and ER protein in these areas. In summary, there is cell-specific regulation of PR by P in the hypothalamus and pituitary, where P down-regulates PR in the pituitary without affecting ER. However, P has no significant effect on PR expression in the hypothalamus even though P decreases ER in the ventromedial nucleus. Although these observations suggest diverse cell-specific regulatory mechanisms, they are consistent with ER- and PR-mediated physiological events, such as PRL secretion and sexual behavior.

    Original languageEnglish (US)
    Pages (from-to)4372-4383
    Number of pages12
    JournalEndocrinology
    Volume137
    Issue number10
    DOIs
    StatePublished - 1996

    Fingerprint

    Progesterone Receptors
    Estrogen Receptors
    Hypothalamus
    Haplorhini
    Steroids
    RNA
    Messenger RNA
    Capsules
    Proteins
    Macaca mulatta
    Middle Hypothalamus
    Arcuate Nucleus of Hypothalamus
    Therapeutics
    Sexual Behavior
    In Situ Hybridization
    Progesterone
    Estradiol
    Down-Regulation
    Immunohistochemistry
    Hormones

    ASJC Scopus subject areas

    • Endocrinology
    • Endocrinology, Diabetes and Metabolism

    Cite this

    Steroid regulation of estrogen and progestin receptor messenger ribonucleic acid in monkey hypothalamus and pituitary. / Bethea, Cynthia; Brown, N. A.; Kohama, Steven.

    In: Endocrinology, Vol. 137, No. 10, 1996, p. 4372-4383.

    Research output: Contribution to journalArticle

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