Steroid receptor coactivator-1 interacts with the p50 subunit and coactivates nuclear factor κB-mediated transactivations

Soon Young Na, Soo-Kyung Lee, Su Ji Han, Hueng Sik Choi, Suhn Young Im, Jae Lee

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Abstract

Steroid receptor coactivator-1 (SRC-1) specifically bound to the transcription factor NFκB subunit p50 but not to p65 as demonstrated by the yeast two hybrid tests and glutathione S-transferase pull down assays. The p50-binding site was localized to a subregion of SRC-1 (amino acids 759- 1141) that encompasses the previously described CBP-p300-binding domain. In mammalian cells, SRC-1 potentiated the NFκB-mediated transactivations in a dose-dependent manner. Coexpression of p300 further enhanced this SRC-1- potentiated level of transactivations, consistent with the recent findings in which CBP and p300 were shown to be transcription coactivators of the p65 subunit (Perkins, N. D., Felzien, L. K., Betts, J. C., Leung, K., Beach, D. H., and Nabel, G. J. (1997) Science 275, 523-527; Gerritsen, M. E., Williams, A. J., Neish, A- S., Moore, S., Shi, Y., and Collins, T. (1997) Proc. Acad. Natl. Sci. U. S. A. 94, 2927-2932). These results suggest that at least two distinct coactivator molecules may cooperate to regulate the NFκB-dependent transactivations in vive and SRC-1, originally identified as a coactivator for the nuclear receptors, may constitute a more widely used coactivation complex.

Original languageEnglish (US)
Pages (from-to)10831-10834
Number of pages4
JournalJournal of Biological Chemistry
Volume273
Issue number18
DOIs
StatePublished - May 1 1998
Externally publishedYes

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Nuclear Receptor Coactivator 1
Transcriptional Activation
Nuclear Receptor Coactivators
Transcription
Glutathione Transferase
Yeast
Assays
Transcription Factors
Yeasts
Binding Sites
Cells
Amino Acids
Molecules

ASJC Scopus subject areas

  • Biochemistry

Cite this

Steroid receptor coactivator-1 interacts with the p50 subunit and coactivates nuclear factor κB-mediated transactivations. / Na, Soon Young; Lee, Soo-Kyung; Han, Su Ji; Choi, Hueng Sik; Im, Suhn Young; Lee, Jae.

In: Journal of Biological Chemistry, Vol. 273, No. 18, 01.05.1998, p. 10831-10834.

Research output: Contribution to journalArticle

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